Arabidopsis Protocols

Handling Arabidopsis Plants: Growth, Preservation of Seeds, Transformation and Genetic Crosses.- Using Arabidopsis Related Model Species (ARMS): Growth, Genetic Transformation and Comparative Genomics.- Growing Arabidopsis in vitro: Cell Suspensions, in vitro Culture, and Regeneration.- Arabidopsis Database and Stock Resources.- Bioinformatic Tools in Arabidopsis Research.- Exploiting Natural Variation in Arabidopsis.- Grafting in Arabidopsis.- Agrobacterium tumefaciens Mediated Transient Transformation of Arabidopsis thaliana Leaves.- iTilling: Personalized Mutation Screen.- Tailor Made Mutations in Arabidopsis Using Zinc-Finger Nucleases.- The Use of Artificial Micro RNA Technology to Control Gene Expression in Arabidopsis thaliana.- Generation and Identification of Arabidopsis EMS Mutants.- Generation and Identification of Arabidopsis T-DNA Insertion Mutants.- Identification of EMS-induced Causal Mutations in Arabidopsis thaliana by Next-Generation Sequencing.- Arabidopsis Transformation with Large Bacterial Artificial Chromosomes.- Global DNA Methylation Analysis Using Methyl-Sensitive Amplification Polymorphism (MSAP).- Next Generation Mapping of Genetic Mutations Using Bulk Population Sequencing.- Chemical Fingerprinting of Arabidopsis Using Fourier-transform Infrared (FT-IR) Spectroscopic Approaches.- A Pipeline for 15N Metabolic Labeling and Phosphoproteome Analysis in Arabidopsis thaliana.- Gene Expression Profiling Using DNA Microarrays.- Forward Chemical Genetic Screening.- Highly-Reproducible ChIP-On-Chip Analysis to Identify Genome-Wide Protein Binding and Chromatin Status in Arabidopsis thaliana.- Fluorescence Microscopy.- Immunocytochemical Fluorescent In Situ Visualization of Proteins in Arabidopsis.- High Pressure Freezing and Freeze Substitution of Arabidopsis For Electron Microscopy.- Applications of Fluorescent Marker Proteins in Plant Cell Biology.- Flow Cytometry and Sorting in Arabidopsis.- Live Imaging of Arabidopsis Development.- Arabidopsis Organelle Isolation and Characterisation.- Analysis of Subcellular Metabolite Distributions within Arabidopsis thaliana Leaf Tissue: A Primer for Subcellular Metabolomics.- Hormone Profiling.- Purification of Protein Complexes and Characterization of Protein-Protein Interactions.- Protein Fragment Bimolecular Fluorescence Complementation Analysis for the In vivo Study of Protein-Protein Interactions and Cellular Protein Complex Localizations.- The Split Ubiquitin System for the Analysis of Three-Component Interactions.- RNA-Binding Protein Immunoprecipitation from Whole Cell Extracts.- High Throughput Analysis of Protein-DNA Binding Affinity.