Quantitation of mRNA by Polymerase Chain Reaction
Springer Berlin (Verlag)
978-3-642-79714-9 (ISBN)
Thomas Köhler, Prof. Dr. med. Dr. phil. Dipl.-Psych. Dipl.-Math., ist Privatdozent am Psychologischen Institut der Universität Hamburg sowie Dozent an verschiedenen Ausbildungsinstituten für Psychotherapeuten. Er ist Verfasser zahlreicher Monographien, u.a. zu Psychosomatik, Biopsychologie und biologischen Grundlagen psychischer Störungen.
I Theoretical and Methodical Prerequisites for Using PCR to Quantitate Nucleic Acids.- 1.1 General Aspects and Chances of Nucleic Acid Quantitation by PCR.- 1.2 Design of Suitable Primers and Competitor Fragments for Quantitative PCR.- 1.3 Cloning of Short DNA Fragments and In Vitro Transcription to Generate RNA Standards.- 1.4 Direct Non-lsotopic Sequencing of PCR Products or Standards.- 2Conventional Techniques for mRNA Analysis.- 2.1 Isolation of mRNA.- 2.2 Synthesis of cDNA.- 2.3 Qualitative RT-PCR: Amplification of Synthesized mdr-1 cDNA.- 2.4 Single-Tube RT-PCR.- 2.5 Nonradioactive Determination of PCR Products by Using a DIG-Labeled DNA Probe (Dot Blot).- 2.6 Nonradioactive Northern Blot Hybridization with DIG-Labeled DNA Probes.- 3 Semiquantitative and Quantitative Protocols for Measurement of Nucleic Acids by PCR.- 3.1 Quantitation of mRNA by the ELOSA Technique Using External Standards.- 3.2 Semiquantitative Detection of Viral DNA, e.g. for CMV, by Using the DNA Enzyme Immunoassay (DEIA).- 3.3 HPLC-Analysis of Nucleic Acids.- 3.4 Quantitation of Absolute Numbers of mRNA Copies in a cDNA Sample by Competitive PCR.- Acknowledgment.
"...this technical book will be very useful for all customers of PCR. And they are numerous!... Summarizing: an excellent booklet, which we recommend highly and absolutely necessary to all practicians of PCR products and their applications." Cellular and Molecular Biology
Erscheint lt. Verlag | 29.12.2011 |
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Reihe/Serie | Springer Lab Manuals |
Zusatzinfo | XX, 166 p. 17 illus. |
Verlagsort | Berlin |
Sprache | englisch |
Maße | 155 x 235 mm |
Gewicht | 305 g |
Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Chemie ► Analytische Chemie | |
Schlagworte | Analyse • Biochemical analysis • Biochemische Analyse • Chemical Analysis • Chemische Analyse • DNA • DNA sequencing • enzymes • Hybridization • Klinisch-chemische Analyse • Nucleic acid • Nucleotide • Nukleinsäuren • PCR • Polymerase chain reaction • polymerase Ketten-Reaktion • Quantitative PCR • RNA • sequencing • synthesis • transcription |
ISBN-10 | 3-642-79714-8 / 3642797148 |
ISBN-13 | 978-3-642-79714-9 / 9783642797149 |
Zustand | Neuware |
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