Handbook of Capillary Electrophoresis Applications

I.1 Electroosmotic flow (EOF) and analyte mobilization.- I.2 Separation modes and samples.- References.- One Equipment Systems.- 1 Sample injection.- 2 Quantitative analysis, precision and reproducibility.- 3 Precision and quantitation in capillary electrophoresis.- 4 Column technology.- 5 Electrolyte systems.- 6 Detection.- 7 Recent advances in fluorescence detection technology.- 8 Other systems.- 9 Advanced systems.- 10 Automated fraction collection in capillary electrophoresis.- 11 Comparison with other analytical methods.- Two Biochemistry Applications.- 12 Amino acids.- 13 Sodium dodecyl sulfate (SDS)-non-gel molecular sieving capillary electrophoresis for proteins.- 14 Protein analysis by capillary electrophoresis.- 15 Analysis of erythropoietin by capillary electrophoresis.- 16 Assay of enzymes by capillary electrophoresis.- 17 Capillary electrophoresis-based immunoassays.- 18 Analysis of antibodies by capillary electrophoresis.- 19 Analysis of peptide hormones and model peptides by capillary electrophoresis.- 20 Affinity analysis by capillary electrophoresis.- 21 Analysis of nucleic acids by capillary electrophoresis.- Three Pharmaceutical Science Applications.- 22 Analysis of pharmaceuticals by capillary electrophoresis.- 23 Analysis of medicinal plants: comparison of capillary electrophoresis with high performance liquid chromatography.- 24 Quantitative applications of the resolution of enantiomers by capillary electrophoresis.- 25 Applications of chiral capillary electrophoresis (cyclodextrin-capillary zone electrophoresis; CD-CZE).- 26 Separation of enantiomeric compounds by micelle-mediated capillary electrokinetic chromatography.- 27 Therapeutic drug monitoring by capillary electrophoresis.- Four Bioscience Applications.- 28 Biomedical applications and biological systems.- 29 Analysis of selected common clinical tests by capillary electrophoresis.- 30 Gene analysis and nucleic acid sequencing.- 31 Gene mutation and DNA sequencing.- 32 Analysis of body fluids: urine, blood, saliva and tears.- 33 Toxins associated mainly with uremia and cancer.- Five Ion Analysis Applications.- 34 Capillary electrophoresis of metal complexes.- 35 Metal chelation.- 36 Inorganic ions.- 37 Organic acids and organic ions.- Six Food Analysis Applications.- 38 Food analysis by capillary electrophoresis.- 39 Capillary zone electrophoresis analysis of additives in food samples.- 40 Analysis of underivatized carbohydrates by capillary electrophoresis and electrochemical detection.- Seven Environmental Science Applications.- 41 Hydrocarbons.- 42 Environmental pollutants.- 43 Analysis of synthetic polymers by capillary electrophoretic techniques.- Appendix A Commercially available instrumentation for capillary electrophoresis.- Appendix A1 Overview of commercial instruments T. Wehr and M. Zhu.- A1.1 Introduction.- A1.2 Power supply.- A1.3 Injection.- A1.4 Capillary temperature control.- A1.5 Liquid handling.- A1.6 Autosamplers.- A1.7 Detectors.- A1.7.1 UV-vis absorbance.- A1.7.2 Fluorescence.- A1.7.3 Other detectors.- A1.7.4 On-line coupling with mass spectrometry (MS).- A1.8 Fraction collection.- Appendix A2 The CAPI series of instruments K. Kitagishi.- A2.1 CAPI-3100.- A2.1.1 Specifications.- A2.1.2 Features.- A2.2 CAPI-1000.- A2.2.1 Specifications.- A2.2.2 Features.- Appendix B Troubleshooting K. Kitagishi.- Appendix C Commercially available buffer reagents H. Shintani.