Triple-Helical Nucleic Acids
Springer-Verlag New York Inc.
978-1-4612-8454-3 (ISBN)
The ability of DNA to exist in configurations other than its classical double-stranded form has been known for many years. There has been a spectacular recent surge of interest in these forms, notably in the three-stranded or triple-helical form. Triplex-like nucleic acids are now known to exist in vivo, and may well participate in significant biological processes. Interest in triple-helical nucleic acids has been greatly stimulated by their potential exploitation to control gene expression, serve as tools in genome mapping strategies, etc. The authors have written an encyclopedic introduction to nucleic acid triplexes based on many years of familiarity with the topic. The book includes information on chemistry, conformation, physical properties, applications, and hypotheses about the biological role of triplexes. It pays particular attention to the different methods for investigating these molecules, a feature which will be welcomed by those new to the field.
1. The Discovery of Triple-Stranded Nucleic Acids.- Basic Physicochemical Properties of Nucleic Acids.- Physicochemical Studies of Model Triple-Stranded Structures.- 2. Methods of Triplex Study.- Physical Methods for Triplex Study.- Immunological Methods.- Affinity Methods.- Electron Microscopy.- X-Ray Analysis.- A Short Overview of Experimental Physical Methods for Triplex Studies.- Theoretical Descriptions of the Triplex Structures.- Enzymatic and Chemical Probing of Triplex Structures.- Enzymatic Methods.- Chemical Methods.- A Short Overview of Chemical and Enzymatic Probes.- Site-Directed Agents.- A Short Overview of Site-Directed Agents.- Conclusion.- 3. General Features of Triplex Structures.- Basic Types of Triplexes.- Molecular Details of Triplex Structure.- DNA—RNA Triplexes.- Kinetics of Triplex Formation.- Thermodynamics of Triplexes.- New Variants of Triplex Structure.- Conclusion.- 4. Triplex Recognition.- Extension of Triplex Recognition Schemes.- Modified Oligomer Backbones.- Protein—DNA Interactions and Triplex Formation.- Triplex DNA—Drug Interactions.- Conclusion.- 5. The Forces Participating in Triplex Stabilization.- Triplex Stabilizing Factors.- Possible Interactions Which Favor and Stabilize Triplexes.- Conclusion.- 6. In Vivo Significance of Triple-Stranded Nucleic Acid Structures.- In Vivo Existence of Triplexes.- Possible Biological Roles of Triplexes.- Other Roles of the PyPu Tracts.- Conclusion.- 7. Possible Spheres of Application of Intermolecular Triplexes.- Applications of Intermolecular Triplex Methodology.- Conclusion.- References.
"A thriving new area of research in nucleic acids focuses on triple helical structures in DNA. The authors review what we know about most aspects of these novel structures, covering structural, molecular recognition and physico-chemical aspects in detail. Read the book to learn the structural aspects of triplexes..." Nature
| Zusatzinfo | XIV, 360 p. |
|---|---|
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 155 x 235 mm |
| Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik |
| Naturwissenschaften ► Biologie ► Biochemie | |
| ISBN-10 | 1-4612-8454-6 / 1461284546 |
| ISBN-13 | 978-1-4612-8454-3 / 9781461284543 |
| Zustand | Neuware |
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