Textbook of Assisted Reproductive Techniques Fourth Edition

David K Gardner DPhil is Chair of Zoology at the University of Melbourne, Australia Ariel Weissman MD is a senior physician at the IVF Unit, Department of Obstetrics and Gynecology. Edith Wolfson Medical Center, Holon, and Sackler Faculty of Medicine,Tel Aviv University, Israel Colin M Howles PhD, FRSM is Vice President, Regional Medical Affairs Fertility, Global Business Unit Fertility and Endocrinology, Merck Serono. SA, Geneva, Switzerland Zeev Shoham MD is Director, Reproductive Medicine and Infertility Unit, Department of Obstetrics and Gynecology, at Kaplan Medical Center, Rehovot, Israel

Establishing and Maintaining an IVF Laboratory
Setting up an ART laboratory; Jacques Cohen, Mina Alikani, Antonia Gilligan, and Tim Schimmel
Quality control: Maintaining stability in the laboratory; David H. McCulloh
The ART laboratory: Current standards; Cecilia Sjöblom
Gamete Collection, Preparation and Selection
Evaluation of sperm; Kaylen M. Silverberg and Tom Turner
Sperm preparation techniques; Harold Bourne, Janell Archer, David H. Edgar, and H. W. Gordon Baker
Sperm chromatin assessment; Ashok Agarwal, Igor Tsarev, Juris Erenpreiss, and Rakesh Sharma
Oocyte retrieval and selection; Laura F. Rienzi and Filippo M. Ubaldi
Preparation and evaluation of oocytes for ICSI; Irit Granot and Nava Dekel
Hyaluronic acid binding-mediated sperm selection for ICSI; Gabor Huszar
Intracytoplasmic morphologically selected sperm injection; Monica Antinori
Use of in vitro maturation in a clinical setting: Patient populations and outcomes; Yoshiharu Morimoto, Aisaku Fukuda, and Manabu Satoh
Micromanipulation
Micromanipulation; Frank L. Barnes
Intracytoplasmic sperm injection: Technical aspects; Queenie V. Neri, Devin Monahan, Zev Rosenwaks, and Gianpiero D. Palermo
Assisted hatching; Anna Veiga and Itziar Belil
Human embryo biopsy procedures; Alan R. Thornhill, Christian Ottolini, and Alan H. Handyside
Culture, Selection and Transfer of the Human Embryo
Analysis of fertilization; Thomas Ebner
Culture systems for the human embryo; David K. Gardner and Michelle Lane
Evaluation of embryo quality: Analysis of morphology and quantification of nutrient utilization and the metabolome; Denny Sakkas and David K. Gardner
Evaluation of embryo quality: Time-lapse imaging to assess embryo morphokinesis; Natalia Basile, Juan García-Velasco, and Marcos Meseguer
Evaluation of embryo quality: Proteomic strategies; Mandy Katz-Jaffe
Cryopreservation
The human oocyte: Controlled rate cooling; Andrea Borini and Veronica Bianchi
The human oocyte: Vitrification; Masashige Kuwayama
The human embryo: Slow freezing; Nikica Zaninovic, Richard Bodine, Robert N. Clarke, Sam Jones, Ye Zhen, and Lucinda L. Veeck Gosden
The human embryo: Vitrification; Zsolt Peter Nagy, Ching-Chien Chang, and Gábor Vajta
Diagnosis of Genetic Disease in Preimplantation Embryos
Severe male factor: Genetic consequences and recommendations for genetic testing; Willy Lissens and Katrien Stouffs; Polar body biopsy and its clinical application; Markus Montag
Preimplantation genetic diagnosis for infertility; Dagan Wells and Elpida Fragouli
Genetic analysis of the embryo; Yuval Yaron, Veronica Gold, Sagit Peleg-Schalka, and Mira Malcov
Implantation
The analysis of endometrial receptivity; Tamara Garrido-Gomez, Francisco Domínguez, Maria Ruiz, Felip Vilella, and Carlos Simon
Future Directions and Clinical Applications
Human embryonic stem cells; Rachel Eiges, Michal Avitzour, and Benjamin Reubinoff
Microfluidics in ART: Current progress and future directions; Jason E. Swain, Thomas B. Pool, Shuichi Takayama, and Gary D. Smith
Index