Dendritic Cell Protocols

I: Reviews.- Review of Human DC Subtypes.- Review of Murine Dendritic Cells: Types, Location, and Development.- II: Human DC Protocols.- Isolation of Human Blood DC Subtypes.- Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin.- Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells.- In Vivo Modulation of Gene Expression by Lentiviral Transduction in “Human Immune System” Rag2?/??c ?/? Mice.- Generation of Stable Th1/CTL-, Th2-, and Th17-Inducing Human Dendritic Cells.- Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation.- Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines.- III: Murine Dendritic Cell Methods.- CD8+, CD8?, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand.- Generation of Large Numbers of Pro-DCs and Pre-DCs In Vitro.- The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSF.- Isolation of Common Dendritic Cell Progenitors (CDP) from Mouse Bone Marrow.- The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow Cytometry.- Isolation of Cutaneous Dendritic Cells.- Isolation of Skin Dendritic Cells from Mouse and Man.- Identification and Isolation of Rodent Respiratory Tract Dendritic Cells.- Isolation of Mouse Thymic Dendritic Cell Precursors.- Isolation of Rat Intestinal Lymph DC.- Mouse Models of Viral Infection: Influenza Infection in the Lung.- DCs in Mouse Models of Intracellular Bacterial Infection.- Studying the Function of Dendritic Cells in Mouse Models of Asthma.- Direct Ex Vivo Activation of T cells for Analysis of Dendritic Cells Antigen Presentation.-Methods to Study Pulmonary Dendritic Cell Migration.- Measuring pH, ROS Production, Maturation, and Degradation in Dendritic Cell Phagosomes Using Cytofluorometry-Based Assays.- Adaptive Treg Generation by DCs and Their Functional Analysis.- Generation of Parabiotic Mice for the Study of DC and DC Precursor Circulation.- Defining In Vivo Dendritic Cell Functions Using CD11c-DTR Transgenic Mice.