Chemotaxis

Microfluidic Techniques for the Analysis of Bacterial Chemotaxis.- Prokaryotic Phototaxis.- Photoorientation in Photosynthetic Flagellates.- Dictyostelium Slug Phototaxis.- Electrotaxis and Wound Healing: Experimental Methods to Study Electric Fields as a Directional Signal for Cell Migration.- Chemotropism During Yeast Mating.- Group Migration and Signal Relay in Dictyostelium.- Quantitative Analysis of Distal Tip Cell Migration in C. elegans.- Inflammation and Wound Healing in Drosophila.- Neutrophil Motility In Vivo Using Zebrafish.- Chemotaxis in Neutrophil-Like HL-60 Cells.- Chemokine Receptor Dimerization and Chemotaxis.- Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph Nodes.- Breast Cancer Cell Movement: Imaging Invadopodia by TIRF and IRM Microscopy.- In Vivo Assay for Tumor Cell Invasion.- Quantitative Studies of Neuronal Chemotaxis in 3D.- Assays for Chemotaxis and Chemoattractant-Stimulated TorC2 Activation and PKB Substrate Phosphorylation in Dictyostelium.- Biochemical Responses to Chemoattractants in Dictyostelium: Ligand-Receptor Interactions and Downstream Kinase Activation.- Quantifying In Vivo Phosphoinositide Turnover in Chemotactically Competent Dictyostelium Cells.- In Vivo Measurements of Cytosolic Calcium in Dictyostelium discoideum.- Chemokine Receptor Signaling and HIV Infection.- Spatiotemporal Stimulation of Single Cells Using Flow Photolysis.- Spatiotemporal Regulation of Ras-GTPases During Chemotaxis.- FRAP Analysis of Chemosensory Components of Dictyostelium.- Monitoring Dynamic GPCR Signaling Events Using Fluorescence Microscopy, FRET Imaging, and Single-Molecule Imaging.- Imaging Actin Cytoskeleton Dynamics in Dictyostelium Chemotaxis.- Analysis of Actin Assembly by In Vitro TIRF Microscopy.- Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Living Dictyostelium Cells.- Imaging B-Cell Receptor Signaling by Single-Molecule Techniques.- Light Microscopy to Image and Quantify Cell Movement.- Mathematics of Experimentally Generated Chemoattractant Gradients.- Modeling Spatial and Temporal Dynamics of Chemotactic Networks.- Computational Modeling of Signaling Networks for Eukaryotic Chemosensing.

From the reviews:

“The purpose is to provide an overview of and practical guidance on the diverse methodologies involved in chemotaxis research. … Each chapter includes an introduction to the topic, lists of necessary materials and reagents, laboratory protocol, and notes on troubleshooting and avoiding pitfalls, followed by current references. … This will serve as a valuable resource for students and scientists seeking practical guidance on diverse methodologies in chemotaxis research.” (Omer Iqbal, Doody’s Review Service, April, 2010)