Fluorescence In Situ Hybridization (FISH) - Application Guide (eBook)

Molecular Cytogenetics, Probes and Equipment.- Molecular Cytogenetic Applications in Diagnostics and Research: An Overview.- Molecular Cytogenetics: The Standard FISH and PRINS Procedure.- Generation of Paint Probes by Flow-Sorted and Microdissected Chromosomes.- ISH Probes Derived from BACs, Including Microwave Treatment for Better FISH Results.- PNA-FISH Technique for In Situ Assessment of Aneuploidy.- Oligonucleotide FISH Probes.- Microscopy and Imaging Systems.- Optical Filters for FISH.- FISH With and Without COT1 DNA.- FISH Applications in Different Tissue Preparations.- Pre- and Postnatal Diagnostics and Research on Peripheral Blood, Chorion, Amniocytes, and Fibroblasts.- Prenatal Diagnostics on Uncultured Amniocytes.- Tumorcytogenetic Diagnostics and Research on Uncultured Blood or Bone Marrow and Smears.- Application of FISH to Archival Cytogenetic Slides.- Characterization of Mosaicism in Various Easy-to-Acquire Body Tissues Such as Buccal Smears, Hair Root Cells or Urine.- Characterization of Archived Formalin-Fixed/Paraffin-Embedded or Cryofixed Tissue, Including Nucleus Extraction.- FISH on Sperms, Spermatocytes and Oocytes.- Characterization of Marker Chromosomes.- Multiplex FISH and Spectral Karyotyping.- Tips and Tricks for mFISH.- Simultaneous Fluorescence Immunostaining and FISH.- Color-Changing Karyotyping (CCK), an M-FISH/SKY Alternative.- Three-Color FISH for the Detection of Individual Radiosensitivity.- FISH Banding Techniques.- cenM-FISH Approaches.- Characterization of Cryptic and Epigenetic Aberrations.- Subtelomeric and/or Subcentromeric Probe Sets.- Quantitative DNA Fiber Mapping.- Parental Origin Determination FISH: Pod-FISH.- Interphase FISH and Characterization of the Nuclear Architecture.- Interphase FISH: Detection of Intercellular Genomic Variations and Somatic Chromosomal Mosaicism.- Three-Dimensional Interphase Analysis Enabled by Suspension FISH.- Applications of FISH in Zoology, Botany and Microbiology.- Animal Probes and ZOO-FISH.- FISH Targeting of Chromosomes and Subchromosomal Regions in Yeast.- FISH on Insect Cells Transfected with Heterologous DNA: A Single-Cell-Directed Approach.- FISH on Plant Chromosomes.- FISH in Food Microbiology.- Comparative Genomic Hybridization (CGH) and Array CGH.- Micro-CGH: Microdissection-Based Comparative Genomic Hybridization.- Array CGH: Opening New Horizons.- Internet Links for FISH.- FISH and Chips on the Internet.