Cell Surface Receptors (eBook)
XII, 220 Seiten
Springer US (Verlag)
978-0-387-23080-1 (ISBN)
Cell Surface Receptors: A Short Course on Theory and Methods, 3rd Edition, links theoretical insights into drug-receptor interactions described in mathematical models with the experimental strategies to characterize the biological receptor of interest. The study of receptors has changed considerably over the period of the publication of the three editions of this book. The cloning of several genomes makes it unlikely that preparations of receptors now or in the future will arise from their purification as trace proteins from native tissues, but rather from a myriad of molecular approaches. Nonetheless, understanding the molecular mechanisms and ultimately the in vivo biology of these receptors means that investigators will engage in molecular, cellular and ultimate in vivo strategies. It should be of value to investigators who want to identify, characterize and understand the biology of a receptor of interest.
<
Cell Surface Receptors: A Short Course on Theory and Methods, 3rd Edition, links theoretical insights into drug-receptor interactions described in mathematical models with the experimental strategies to characterize the biological receptor of interest. The study of receptors has changed considerably over the period of the publication of the three editions of this book. The cloning of several genomes makes it unlikely that preparations of receptors now or in the future will arise from their purification as trace proteins from native tissues, but rather from a myriad of molecular approaches. Nonetheless, understanding the molecular mechanisms and ultimately the in vivo biology of these receptors means that investigators will engage in molecular, cellular and ultimate in vivo strategies. It should be of value to investigators who want to identify, characterize and understand the biology of a receptor of interest.
lt;
Introduction to Receptor Theory - Origin of the Receptor Concept - Occupancy Theory -Relationship Between Occupancy and Response - Concept of Spare Receptors - Operational Models of Pharmacological Agonism - Rate Theory - Allosteric Theory - Beyond Two-State Receptor Theory – Summary.- Characterization of Receptors Based on Receptor-Mediated Responses - Characterization of Receptor Specificity - Determining Equilibrium Dissociation Constants (Kd Values) for Receptor-Ligand Interactions Based on Measurements of Receptor-Mediated Response - Pharmacologic Resultant Analysis – Summary.- Identification of Receptors Using Direct Radioligand Binding Techniques – Methods - Data Generation - Criteria Expected for Binding of *D to the Physiologically Relevant Receptor, R - Determination of Rate Constants for Radioligand Association and Dissociation – Summary.- Complex Binding Phenomena - Mathematical Descriptions of Complex Binding Phenomena 124 - Non-Linear Regression Analysis of Complex Binding Phenomena - Independent Receptor Subtypes - Affinity States of a Single Receptor Population – Summary.- The Preparation and Study of Detergent-Solubilized Receptors - General Properties of Biological Membranes and Detergent Micelles - Choice of a Biological Detergent - Solubilizing Receptors from Biological Membranes - Methods for Analysis of Detergent-Solubilized Receptors – Summary.- Quantifying Cell Surface Receptor Binding and Turnover in Intact Cells - Discriminating Between Cell Surface Versus Intracellular Receptor-Ligand Complexes - Biochemical Evidence Consistent with Recycling of Cell Surface Receptors - Assessment of Rate Constants for Receptor Turnover Using a Steady State Mathematical Analysis of Intact Cell Radioligand Binding Data - The Heavy Amino Acid Density-Shift Technique for Quantitating Receptor Synthesis and Turnover – Summary.- Index.
Erscheint lt. Verlag | 2.6.2006 |
---|---|
Zusatzinfo | XII, 220 p. 34 illus. |
Verlagsort | New York |
Sprache | englisch |
Themenwelt | Medizin / Pharmazie ► Gesundheitsfachberufe |
Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie | |
Medizin / Pharmazie ► Pharmazie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Technik | |
Schlagworte | Amino acid • Molecular mechanisms • proteins • termination • tissue |
ISBN-10 | 0-387-23080-7 / 0387230807 |
ISBN-13 | 978-0-387-23080-1 / 9780387230801 |
Haben Sie eine Frage zum Produkt? |
Größe: 11,0 MB
DRM: Digitales Wasserzeichen
Dieses eBook enthält ein digitales Wasserzeichen und ist damit für Sie personalisiert. Bei einer missbräuchlichen Weitergabe des eBooks an Dritte ist eine Rückverfolgung an die Quelle möglich.
Dateiformat: PDF (Portable Document Format)
Mit einem festen Seitenlayout eignet sich die PDF besonders für Fachbücher mit Spalten, Tabellen und Abbildungen. Eine PDF kann auf fast allen Geräten angezeigt werden, ist aber für kleine Displays (Smartphone, eReader) nur eingeschränkt geeignet.
Systemvoraussetzungen:
PC/Mac: Mit einem PC oder Mac können Sie dieses eBook lesen. Sie benötigen dafür einen PDF-Viewer - z.B. den Adobe Reader oder Adobe Digital Editions.
eReader: Dieses eBook kann mit (fast) allen eBook-Readern gelesen werden. Mit dem amazon-Kindle ist es aber nicht kompatibel.
Smartphone/Tablet: Egal ob Apple oder Android, dieses eBook können Sie lesen. Sie benötigen dafür einen PDF-Viewer - z.B. die kostenlose Adobe Digital Editions-App.
Buying eBooks from abroad
For tax law reasons we can sell eBooks just within Germany and Switzerland. Regrettably we cannot fulfill eBook-orders from other countries.
aus dem Bereich