Cell Surface Receptors (eBook)

A Short Course on Theory and Methods
eBook Download: PDF
2006 | 3rd ed. 2005
XII, 220 Seiten
Springer US (Verlag)
978-0-387-23080-1 (ISBN)

Lese- und Medienproben

Cell Surface Receptors -  Lee E. Limbird
Systemvoraussetzungen
149,79 inkl. MwSt
  • Download sofort lieferbar
  • Zahlungsarten anzeigen

Cell Surface Receptors: A Short Course on Theory and Methods, 3rd Edition, links theoretical insights into drug-receptor interactions described in mathematical models with the experimental strategies to characterize the biological receptor of interest. The study of receptors has changed considerably over the period of the publication of the three editions of this book. The cloning of several genomes makes it unlikely that preparations of receptors now or in the future will arise from their purification as trace proteins from native tissues, but rather from a myriad of molecular approaches. Nonetheless, understanding the molecular mechanisms and ultimately the in vivo biology of these receptors means that investigators will engage in molecular, cellular and ultimate in vivo strategies. It should be of value to investigators who want to identify, characterize and understand the biology of a receptor of interest.



<
Cell Surface Receptors: A Short Course on Theory and Methods, 3rd Edition, links theoretical insights into drug-receptor interactions described in mathematical models with the experimental strategies to characterize the biological receptor of interest. The study of receptors has changed considerably over the period of the publication of the three editions of this book. The cloning of several genomes makes it unlikely that preparations of receptors now or in the future will arise from their purification as trace proteins from native tissues, but rather from a myriad of molecular approaches. Nonetheless, understanding the molecular mechanisms and ultimately the in vivo biology of these receptors means that investigators will engage in molecular, cellular and ultimate in vivo strategies. It should be of value to investigators who want to identify, characterize and understand the biology of a receptor of interest.

lt;

Introduction to Receptor Theory - Origin of the Receptor Concept - Occupancy Theory -Relationship Between Occupancy and Response - Concept of Spare Receptors - Operational Models of Pharmacological Agonism - Rate Theory - Allosteric Theory - Beyond Two-State Receptor Theory – Summary.- Characterization of Receptors Based on Receptor-Mediated Responses - Characterization of Receptor Specificity - Determining Equilibrium Dissociation Constants (Kd Values) for Receptor-Ligand Interactions Based on Measurements of Receptor-Mediated Response - Pharmacologic Resultant Analysis – Summary.- Identification of Receptors Using Direct Radioligand Binding Techniques – Methods - Data Generation - Criteria Expected for Binding of *D to the Physiologically Relevant Receptor, R - Determination of Rate Constants for Radioligand Association and Dissociation – Summary.- Complex Binding Phenomena - Mathematical Descriptions of Complex Binding Phenomena 124 - Non-Linear Regression Analysis of Complex Binding Phenomena - Independent Receptor Subtypes - Affinity States of a Single Receptor Population – Summary.- The Preparation and Study of Detergent-Solubilized Receptors - General Properties of Biological Membranes and Detergent Micelles - Choice of a Biological Detergent - Solubilizing Receptors from Biological Membranes - Methods for Analysis of Detergent-Solubilized Receptors – Summary.- Quantifying Cell Surface Receptor Binding and Turnover in Intact Cells - Discriminating Between Cell Surface Versus Intracellular Receptor-Ligand Complexes - Biochemical Evidence Consistent with Recycling of Cell Surface Receptors - Assessment of Rate Constants for Receptor Turnover Using a Steady State Mathematical Analysis of Intact Cell Radioligand Binding Data - The Heavy Amino Acid Density-Shift Technique for Quantitating Receptor Synthesis and Turnover – Summary.- Index.

Erscheint lt. Verlag 2.6.2006
Zusatzinfo XII, 220 p. 34 illus.
Verlagsort New York
Sprache englisch
Themenwelt Medizin / Pharmazie Gesundheitsfachberufe
Medizin / Pharmazie Medizinische Fachgebiete Pharmakologie / Pharmakotherapie
Medizin / Pharmazie Pharmazie
Naturwissenschaften Biologie Zellbiologie
Technik
Schlagworte Amino acid • Molecular mechanisms • proteins • termination • tissue
ISBN-10 0-387-23080-7 / 0387230807
ISBN-13 978-0-387-23080-1 / 9780387230801
Haben Sie eine Frage zum Produkt?
PDFPDF (Wasserzeichen)
Größe: 11,0 MB

DRM: Digitales Wasserzeichen
Dieses eBook enthält ein digitales Wasser­zeichen und ist damit für Sie persona­lisiert. Bei einer missbräuch­lichen Weiter­gabe des eBooks an Dritte ist eine Rück­ver­folgung an die Quelle möglich.

Dateiformat: PDF (Portable Document Format)
Mit einem festen Seiten­layout eignet sich die PDF besonders für Fach­bücher mit Spalten, Tabellen und Abbild­ungen. Eine PDF kann auf fast allen Geräten ange­zeigt werden, ist aber für kleine Displays (Smart­phone, eReader) nur einge­schränkt geeignet.

Systemvoraussetzungen:
PC/Mac: Mit einem PC oder Mac können Sie dieses eBook lesen. Sie benötigen dafür einen PDF-Viewer - z.B. den Adobe Reader oder Adobe Digital Editions.
eReader: Dieses eBook kann mit (fast) allen eBook-Readern gelesen werden. Mit dem amazon-Kindle ist es aber nicht kompatibel.
Smartphone/Tablet: Egal ob Apple oder Android, dieses eBook können Sie lesen. Sie benötigen dafür einen PDF-Viewer - z.B. die kostenlose Adobe Digital Editions-App.

Buying eBooks from abroad
For tax law reasons we can sell eBooks just within Germany and Switzerland. Regrettably we cannot fulfill eBook-orders from other countries.

Mehr entdecken
aus dem Bereich