NMR Spectroscopy and its Application to Biomedical Research (eBook)

Front Cover 1
NMR Spectroscopy and its Application to Biomedical Research 4
Copyright Page 5
Contents 10
Preface 8
Foreword 16
List of contributors 18
Chapter 1. Two-Dimensional NMR Spectroscopy: A Graphical, "Top-Down" Description 20
1. Introduction 20
2. Coherence flow networks 22
3. Phase cycling and pulsed field gradients 35
4. Two-dimensional NMR 55
5. Coherence transfer 65
6. Homonuclear correlated spectroscopy (COSY) 71
7. Multiple quantum filtered spectroscopy 80
8. Isotropic homonuclear coherence transfer 84
9. Incoherent magnetization transfer 87
10. Heteronuclear shift correlations 88
11. Conclusion 101
References 101
Chapter 2. Multidimensional NMR of Macromolecules 104
1. Introduction 104
2. Principles ofmultidimensional NMR spectroscopy 106
3. Delineationof inter-nuclear connectivity networks 109
4. Assignment strategies 137
5. Coupling constants 141
6. Dynamic processes 145
7. Toolboxof pulse sequence design 148
8. Hardware requirements 156
9. Outlook and concluding remarks 158
References 160
Chapter 3. Preparation of 2H, 13C and 15N Isotopically-enriched Proteins for NMR Spectroscopic Investigations 178
1. Introduction 178
2. Isotopic enrichment methodologies 183
3. Determination of enrichment level 194
4. NMR spectroscopy of isotopically enriched proteins 194
5. Future perspectives 195
References 196
Chapter 4. Protein Structure Determination from NMR Data 206
1. Introduction 206
2. Determining resonance assignments 209
3. Generating experimental constraints 215
4. Structure calculation strategies 226
5. Analysis ofthe calculated structures 241
6. Future prospects 248
7. Conclusion 255
References 255
Chapter 5. Protein Dynamics as Studied by Solution NMR Techniques 260
1. Introduction 260
2. Theory 262
3. Experimental techniques and data analysis 269
4. Experimental applications 280
5. Conclusions 293
References 295
Chapter 6. Nucleic Acid Structure and Dynamics from NMR 300
1. Introduction 300
2. Resonance assignments in nucleic acids 301
3. Parameters for structure determination 309
4. Developing models 313
5. Analysis of dynamics 316
6. Examples of structuredeterminations 319
7. Summary 327
References 327
Chapter 7. Carbohydrate Structure and Dynamics from NMR 332
1. Introduction 332
2. Assignment 335
3. Conformation 344
4. Analysis of glycoconjugates 348
5. Interactions of oligosaccharides 353
6. Future directions 354
References 355
Chapter 8. Solid State NMR and its Applications to Biomedical Research 364
1. Introduction 364
2. Fundamentals of solid-state NMR 365
3. Applications ofsolid-state NMR to questions of biomedical interest 377
4. Conclusion 395
References 396
Subject index 400