Biophotonics, Part A (eBook)

Cover 1
Title Page 3
Copyright Page 5
Table of Contents 6
Contributors to Volume 360 10
Preface 14
Volumes in Series 16
Chapter 1. Fluorescence: Basic Concepts, Practical Aspects, and Some Anecdotes 38
Chapter 2. Fluorescence-Sensing Methods 81
Chapter 3. Bioluminescence and Chemiluminescence 112
Chapter 4. Fluorescent Amino Acid Analogs 141
Chapter 5. Fluorescent Nucleotides: Synthesis and Characterization 165
Chapter 6. Photophysics of Green and Red Fluorescent Proteins: Implications for Quantitative Microscopy 215
Chapter 7. Development of Genetically Encoded Fluorescent Indicators for Calcium 239
Chapter 8. Development and Application of Caged Calcium 263
Chapter 9. Application of Fluorescent Probes to Study Mechanics and Dynamics of Ca2+-Triggered Synaptotagmin C2 Domain–Membrane Interactions 275
Chapter 10. Caging Proteins through Unnatural Amino Acid Mutagenesis 295
Chapter 11. Preparation and Light-Directed Activation of Caged Proteins 311
Chapter 12. Bioluminescence Resonance Energy Transfer: Monitoring Protein–Protein Interactions in Living Cells 326
Chapter 13. Structure–Function Relationships in Metalloproteins 339
Chapter 14. Spectroscopy and Microscopy of Cells and Cell Membrane Systems 367
Chapter 15. Photonics for Biologists 382
Chapter 16. Imaging at Low Light Levels with Cooled and Intensified Charge-Coupled Device Cameras 420
Chapter 17. Filters and Mirrors for Applications in Fluorescence Microscopy 431
Chapter 18. Resolution in Optical Microscopy 453
Chapter 19. Video-Rate Confocal Microscopy 484
Chapter 20. Giant Vesicles, Laurdan, and Two-Photon Fluorescence Microscopy: Evidence of Lipid Lateral Separation in Bilayers 518
Chapter 21. Biological Near-Field Microscopy 538
Chapter 22. Fluorescence Lifetime-Resolved Imaging: Measuring Lifetimes in an Image 546
Chapter 23. Fluorescence Resonance Energy Transfer Imaging Microscopy 579
Chapter 24. Fluorescence Resonance Energy Transfer Imaging Microscopy and Fluorescence Polarization Imaging Microscopy 598
Chapter 25. Homo-FRET versus Hetero-FRET to Probe Homodimers in Living Cells 617
Chapter 26. Spinning Disk Confocal Microscope System for Rapid High-Resolution, Multimode, Fluorescence Speckle Microscopy and Green Fluorescent Protein Imaging in Living Cells 634
Chapter 27. Single-Particle Tracking Image Microscopy 655
Chapter 28. Diffusion in Cells Measured by Fluorescence Recovery after Photobleaching 672
Chapter 29. Fluorophore-Assisted Light Inactivation for Multiplex Analysis of Protein Function in Cellular Processes 686
Author Index 698
Subject Index 736