Superoxide Dismutase (eBook)

Cover 1
Table of Contents 6
Contributors to Volume 349 10
Preface 16
Volume in Series 18
Section I: Superoxide Reactions and Mechanisms 38
Chapter 1. Quantitation of Intracellular Free Iron by Electron Paramagnetic Resonance Spectroscopy 40
Chapter 2. Aconitase: Sensitive Target and Measure of Superoxide 46
Chapter 3. Reactions of Manganese Porphyrins and Manganese-Superoxide Dismutase with Peroxynitrite 60
Chapter 4. Superoxide Dismutase Kinetics 75
Chapter 5. Analysis of Cu, ZnSOD Conformational Stability by Differential Scanning Calorimetry 86
Chapter 6. Catalytic Pathway of Manganese Superoxide Dismutase by Direct Observation of Superoxide 98
Chapter 7. Extracellular Superoxide Dismutase 111
Chapter 8. Prokaryotic Manganese Superoxide Dismutases 117
Chapter 9. Nickel-Containing Superoxide Dismutase 127
Chapter 10. Reversible Conversion of Nitroxyl Anion to Nitric Oxide 138
Chapter 11. Purification and Determination of Activity of Mitochondrial Cyanide-Sensitive Superoxide Dismutase in Rat Tissue Extract 143
Chapter 12. Studies of Metal-Binding Properties of Cu,Zn Superoxide Dismutase by Isothermal Titration Calorimetry 152
Chapter 13. Superoxide Reductase from Desulfoarculus baarsii 160
Chapter 14. Enzyme-Linked Immunosorbent Assay for Human and Rat Manganese Superoxide Dismutases 166
Section II: Mutants, Knockouts, Transgenics 180
Chapter 15. Investigating Phenotypes Resulting from a Lack of Superoxide Dismutase in Bacterial Null Mutants 182
Chapter 16. Bacterial Superoxide Dismutase and Virulence 192
Chapter 17. Superoxide Dismutase Null Mutants of Baker’s Yeast, Saccharomyces cerevisiae 204
Chapter 18. Measurement of FreeŽ or Electron Paramagnetic Resonance-Detectable Iron in Whole Yeast Cells as Indicator of Superoxide Stress 210
Chapter 19. Transgenic Superoxide Dismutase Overproducer: Murine 217
Chapter 20. Transgenic and Mutant Mice for Oxygen Free Radical Studies 228
Chapter 21. Overexpression of Cu,ZnSOD and MnSOD in Transgenic Drosophila 250
Section III: Superoxide Dismutase Mimics 258
Chapter 22. Manganese Porphyrins and Related Compounds as Mimics of Superoxide Dismutase 260
Chapter 23. Superoxide Dismutase Mimics: Antioxidative and Adverse Effects 271
Chapter 24. Superoxide Reductase Activities of Neelaredoxin and Desulfoferrodoxin Metalloproteins 280
Chapter 25. Purification and Preparation of Prion Protein: Synaptic Superoxide Dismutase 295
Section IV: In Vivo Sources, Cell Signaling 306
Chapter 26. Mitochondrial Superoxide Anion Production and Release into Intermembrane Space 308
Chapter 27. Measurement of Superoxide Radical and Hydrogen Peroxide Production in Isolated Cells and Subcellular Organelles 317
Chapter 28. Biochemical Assay of Superoxide Dismutase Activity in Drosophila 324
Chapter 29. Transcriptional Regulation and Environmental Induction of Gene Encoding Copper- and Zinc- Containing Superoxide Dismutase 330
Chapter 30. Transcription Regulation of Human Manganese Superoxide Dismutase Gene 343
Chapter 31. Assessment of Oxidants in Mitogen-Activated Protein Kinase Activation 350
Chapter 32. Assaying Binding Capacity of Cu,ZnSOD and MnSOD: Demonstration of Their Localization in Cells and Tissues 358
Section V: Superoxide Dismutase in Aging and Disease Therapy 366
Chapter 33. Superoxide Dismutase in Aging and Disease: An Overview 368
Chapter 34. Tissue-Specific Mitochondrial Production of H2O2: Its Dependence on Substrates and Sensitivity to Inhibitors 378
Chapter 35. Targeting Superoxide Dismutase to Critical Sites of Action 383
Chapter 36. InVitro Quantitation of Biological Superoxide and Hydrogen Peroxide Generation 391
Author Index 400
Subject Index 428