Standardization and Quality Assurance in Fluorescence Measurements I (eBook)

Need for Standardization of Fluorescence Measurements from the Regulator´s View.- Need for and Metrological Approaches towards Standardization of Fluorescence Measurements from the View of National Metrological Institutes.- Linking Fluorometry to Radiometry: Traceability and Physical and Fluorescence Standards.- Fluorescence Quantum Yield: Traceability, Methods of Determination and Standards.- Long Wavelength and Near-IR Fluorescence: the State-of-the-Art and the Need for Standards.- Surface Fluorescence: The Only Standardized Method of Measuring Luminescence.- Time Resolved Fluorometry: Typical Methods, Challenges, Applications and Standards.- Practical Time-Resolved Fluorescence Spectroscopy: Avoiding Artifacts and Using Lifetime Standards.- Evaluation of Time Resolved Fluorescence Data: Typical Methods and Problems.- Time Resolved Fluorescence: Novel Technical Solutions and Future Need for Standards.- Fluorescence Depolarization Techniques in Materials Sciences.- Fluorescence Polarization Techniques: Applications, Pitfalls and Future Trends.- Introduction: Classification of Chemical Sensing.- Fiber-Optic and Nanoparticle-Based Fluorescence Sensing: Pitfalls, Self- Referencing, Application, and Future Trends.- Intrinsically Referenced Fluorometric Detection Schemes: Advantages and Applications.- TIRF Sensing, Quality Assurance and Application to Water Analysis.- Fluorescence Sensing and Imaging Using PSPs and TSPs.- Introduction to Fluorescence Microscopy: Methods and Instrumentation, Quality Assurance and Standards.- Need for Standardization of Fluorescence Measurements from the Instrument Manufacturer´s View.- Approaches to the Characterization of Fluorescence Microscopes.- Quantification in Fluorescence Microscopy.- Confocal Spectral Imaging Systems: Instrument Characterization and Comparability of Data.- Fluorescence Lifetime Imaging: Quality Assurance and Standards.- State-of-the-Art and Novel Trends in Fluorescence Correlation Spectroscopy.- SingleMolecule Spectroscopy: Instrumentation and Multiparameter Detection.- DNA-Microarrays: Applications, Future Trends, and Need for Standardization.- Comparability of Microarray Experiments from the Instrument and the Sample Site and Approaches Towards Standardization.- Microarray Technology: Unresolved Issues and Future Challenges from the Regulator´s View.- Protein Microarrays and Fluorescence Detection: Applications and Limitations.- Flow Cytometry: Instrumentation, Applications, Future Trends and Limitations.- Flow Cytometry Quality Assurance.- Approaches to Quantification in Flow Cytometry.- Immunoassays: Basic Concepts, Physical Chemistry and Validation.- Time-Resolved Fluorescence Immunofluorometric Assays: Instrumentation, Applications, Unresolved Issues and Future Trends.- Particle-Based Assays: Applications and Unresolved Issues.- Recent Advances in Fluorescence Enzyme Detection Methods.- Quantitative PCR: Applications, PitFalls and Unresolved Issues.- High Throughput, Quantitive Measurements In Fluorescence In Situ Hybridization and Immunohistochemistry: Standards, Technology and Applications.- Fluorescence Analysis of Actinides: Instrumentation, Applications, Quantification, Future Trends and Quality Assurance.- Fluorescence Technologies in Biomedical Diagnostics: Instrumentation, Applications and Unresolved Issues.- In vivo Fluorescence Imaging: Applications, Future Trends & Approaches to Standardization