Replacing Animal Models – A Practical Guide to Creating and Using Culture–based Biomimetic Alternatives

Professor Jamie A. Davies, Centre for Integrative Physiology, Hugh Robson Building, George Square, University of Edinburgh, Edinburgh.

Contributors, vii Preface, xi Section 1 Introductory Material 1 Potential Advantages of Using Biomimetic Alternatives, 3 Jamie Davies 2 Overview of Biomimetic Alternatives, 13 Jamie Davies Section 2 Culture Methods 3 Pancreatic Islets, 23 Eli C Lewis 4 Endometrial Organoid Culture, 35 Merja Blauer 5 Modelling Lymphatic and Blood Capillary Patterning, 45 Francoise Bruyere, Catherine Maillard, Charlotte Erpicum and Agnes Noel 6 Precision-cut Lung Slices (PCLS), 57 Christian Martin and Stefan Uhlig 7 Human Colon Tissue in Organ Culture, 69 Michael K Dame and James Varani 8 Fetal Organ Culture, 81 Jamie Davies 9 Design of a Mechanical Loading Device to Culture Intact Bovine Spinal Motion Segments under Multiaxial Motion, 89 Jochen Walser, Stephen John Ferguson and Benjamin Gantenbein-Ritter 10 Magnetic Assembly of Tissue Surrogates, 107 Chien-Yu Fu and Hwan-You Chang 11 Assembly of Renal Tissues by Cellular Self-organization, 115 Mathieu Unbekandt Section 3 Case Studies of Use 12 Hierarchical Screening of Pathways: Using Cell and Organ Cultures to Reduce use of Transgenic Mice, 125 Guangping Tai and Jamie Davies 13 Lung Organoid Culture to Study Responses to Viruses, 137 Wenxin Wu, J Leland Booth and Jordan P Metcalf 14 Organ-cultured Human Skin for the Study of Epithelial Cell Invasion of Stroma, 151 James Varani 15 Organotypic Mandibular Cultures for the Study of Inflammatory Bone Pathology, 159 Alastair J Sloan, Sarah Y Taylor and Emma L Smith 16 Three-dimensional, High-density and Tissue Engineered Culture Models of Articular Cartilage, 167 Ali Mobasheri, Sara Kelly, Abigail L. Clutterbuck, Constanze Buhrmann and Mehdi Shakibaei 17 Concluding Remarks, 193 Jamie Davies Appendix 1 Sources of funding for development of culture-based alternatives, 195 Appendix 2 Databases and web-based discussions relevant to development of alternatives, 197 Index, 199