Modern Clinical Molecular Techniques

Preface.- Acknowledgement.- Contributors.- Specimen Collection, Handling and Processing.- DNA/RNA Isolation and Quantitation.- Test Validation.- In Vitro Amplification Methods in Molecular Diagnostics.-Whole Genome Sequencing in the Clinical Laboratory.-Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction.- Reporting Clinical Molecular Genetic Laboratory Results.- HIV-1 Genotyping and Virtual Phenotyping.- COBAS® Ampliprep/Taqman HIV-1 Test.- Hepatitis C Virus (HCV) Genotype Assay (LiPA).- COBAS® Ampliprep/Taqman HCV Test.- Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction.-Quantification of BK Viral Load.-Molecular Diagnosis and Epidemiology of Rabies.- Pyrosequencing for Bacterial Identification.-Other Pathogens of Significant Public Health Concern.- Gene Rearrangements Testing.-Mutation Analysis in Cancer.-Minimal Residual Disease.- Pyrosequencing in Cancer.- Clinical Applications of Microarrays in Cancer.-Basic Theories and Clinical Applications of Molecular Flow Cytometry.-Molecular Prenatal Diagnostic Testing.- PCR Based Diagnosis of Fragile X Syndrome.- Microsatellite Testing Short Tandem Repeat Profile Generation.- Parentage.-Diagnostic Molecular Training.- Molecular Diagnostics Point of Care Testing Training.