mTOR

Mammalian Target of Rapamycin: A Signaling Kinase for every Aspect of Cellular Life.-Biochemical and Pharmacological Inhibition of mTOR by Rapamycin and an ATP-Competitive mTOR Inhibitor.-Evaluation of the Nutrient-Sensing mTOR Pathway.-mTOR Activity Under Hypoxia.-Isolation of the mTOR Complexes by Affinity Purification.-An in vitro Assay for the Kinase Activity of mTOR Complex 2.-Overexpression or Downregulation of mTOR in Mammalian Cells.-Detection of Cytoplasmic and Nuclear Functions of mTOR by Fractionation.-Evaluation of Rapamycin-Induced Cell Death.-Evaluation of mTOR-Regulated mRNA Translation.-A Genome-wide RNAi Screen for Polypeptides that Alter rpS6 Phosphorylation.-Immunohistochemical Analysis of mTOR activity in Tissues.-Assessing Cell Size and Cell Cycle Regulation in Cells With Altered TOR Activity.-Quantitative Visualization of Autophagy Induction by mTOR Inhibitors.-The in vivo Evaluation of Active-Site TOR Inhibitors (asTORi) in Models of BCR-ABL+ Leukemia.-Inducible raptor and rictor Knockout Mouse Embryonic Fibroblasts.-Expanding Human T Regulatory Cells with the mTOR-Inhibitor Rapamycin.-Rapamycin Induced Enhancement of Vaccine Efficacy in Mice.-Utilizing a Retroviral RNAi System to Investigate in vivo mTOR Functions in T Cells.-Exploring Functional in Vivo Consequences of the Selective Genetic Ablation of mTOR Signaling in T Helper Lymphocytes.-Evaluating the Therapeutic Potential of mTOR Inhibitors Using Mouse Genetic.- Inhibition of PI3K-Akt-mTOR Signaling in Glioblastoma by mTORC1/2 Inhibitors.-Assessing the Function of mTOR in Human Embryonic Stem Cells.-Video-EEG Monitoring Methods for Characterizing Rodent Models of Tuberous Sclerosis and Epilepsy.-A Genetic Model to Dissect the Role of Tsc-mTORC1 in Neuronal Cultures.-Tissue-Specific Ablation of Tsc1 in Pancreatic Beta-Cells.-A Mouse Model of Diet-Induced Obesity and Insulin Resistance.-Rapamycin as Immunosuppressant in Murine Transplantation Model.-Development of ATP-Competitive mTOR Inhibitors.