Molecular Protocols in Transfusion Medicine

Preface
List of Abbreviations
Part 1: Introduction
1.1 Background
1.2 Aim, Scope and Purpose of the Book
1.3 Organization of the Book
1.4 Terminology
1.5 Regulatory Compliance
1.6 References
Part 2: General Considerations
2.1 Clinical Information
2.2 Basic Principles of Molecular Analysis
2.3 Troubleshooting
2.4 References
Part 3: Protocols
3.1 Sample Shipment and Storage
3.2 Reagents
3.3 Harvesting WBCs by RBC Sedimentation
3.4 Short-term Cell Cultures from Amniotic Fluid
3.5 Cultured Amniocyte Cell Harvesting
3.6 Cell Preparation and DNA Extraction
3.7 Assessment of DNA Quality, Quantity and Purity
3.8 PCR Amplification
3.9 Restriction Enzyme Digestion for PCR-RFLP Analysis
3.10 Allele-specific PCR
3.11 Agarose Gel Preparation and Electrophoresis
3.12 Acrylamide Gel Preparation and Electrophoresis
3.13 Single-strand Conformation Polymorphism (SSCP)
3.14 Optimizing a PCR
3.15 Gene Zygosity Assessment by Quantitative PCR Amplification
3.16 VNTR and STR Analysis by PCR Amplification
3.17 Multiplex PCR Analysis
3.18 Southern Analysis
3.19 References
Part 4: Red Cell Blood Groups
4.1 Terminology
4.2 Clinical Applications
4.3 Situations where Genotype and Phenotype do not Agree
4.4 References
4.5 Facts Sheets, Gene Maps and Molecular Protocols
Part 5: Platelet Blood Groups
5.1 Terminology
5.2 Clinical Applications
5.3 References
5.4 Facts Sheets, Gene Maps and Molecular Protocols
Part 6: Neutrophil Blood Groups
6.1 Terminology
6.2 Clinical Applications
6.3 References
6.4 Facts Sheets, Gene Maps and Molecular Protocols
Part 7: Appendices
7.1 Database Information
7.2 Glossary
7.3 References
7.4 Works Sheets and Request Forms
Index
Useful Tables