Mammalian Cell Culture

1 Serum Factor Requirements for the Initiation of Cellular Proliferation.- Multiple Serum Components Required for Cellular Proliferation.- EOF and Somatomedin C Can Replace the GQ/GI Progession Activity of PPP.- EGF and Somatomedin C Reduce the Minimum Gi Transit Time.- Availability of Free Somatomedin C Controls Minimum Gi Transit Time.- Somatomedin C Regulates Progression of Late Gi Phase and Commitment to DNA Synthesis.- EGF Is Required during Traverse of Early Gi.- Summary and Conclusions.- References.- 2 Regulation of Fibroblast Growth by Multiple Growth Factors in Serum-Free Medium.- In Vitro Models of Growth Regulation-Why Do Cells Arrest Their Growth?.- Neoplastic Transformation and Relaxed Growth Regulation.- Replacing Serum with Defined Medium Components to Study Growth Regulation In Vitro.- Adhesion-Promoting Components.- Nutrients and Trace Minerals.- Transport Proteins.- Factors That Stimulate DNA Synthesis.- Combinations of Growth Factors Are Required in Defined Media to Support Cell Proliferation.- Mechanism of Growth Factor Action-Events Stimulated by Growth Factors Prior to DNA Synthesis.- Comparison of the Growth Factor Requirements of Normal and Transformed Cells Grown in Serum-Free Media.- Conclusion.- References.- 3 Growth and Differentiation of Preadipocyte Cell Lines in Serum-Free Medium.- In Vitro Models of Adipocytelike Cells.- Differentiation Program.- Factors Influencing Adipose Conversion in Vitro.- Results and Discussion.- Isolation of the Teratoma-Derived Adipogenic Cell Line, 1246.- Growth of 1246 Cells in Serum-Free Medium.- Differentiation of 1246 Cells.- Regulator versus Accelerator of Differentiation.- Insulin-Independent Variant 1246-3A.- References.- 4 Growth and Differentiation of Human Myelomonocyte Leukemia Cell Lines in Serum-Free Medium.- Human Myelomonocytic Cell Lines.- Growth of HL-60 in Defined Medium.- Differentiation of HL-60 in Defined Medium.- Involvement of Intracellular Cyclic Adenosine 3?:5?-Monophosphate (cAMP) in Differentiation of HL-60.- Differentiation Effects of Agents That Increase Intracellular cAMP on HL-60 Primed with RA.- Growth and Differentiation of Other Myelomonocytic Cell Lines in Defined Medium.- References.- 5 In Vitro Immunization and Growth of Hybridomas in Serum-Free Medium.- In Vitro Immunization.- The Role of Serum.- Thiols.- Cellular Interactions and Soluble Factors.- Serum-Free Media.- Conclusion.- References.- 6 Kidney Cell Cultures in Hormonally Defined Serum-Free Medium.- Kidney Cell Culture in Serum-Supplemented Medium: MDCK and LLC-PKl as Model Systems.- Advantages of Kidney Cell Culture.- Limitations on Transport Studies with Kidney Cell Culture Imposed by Serum.- Limitations of Studies with Established Kidney Cell Lines: Importance of Primary Kidney Cell Culture.- Primary Kidney Culture and Hormonally Defined Medium.- Primary Culture of Specific Kidney Cell Types.- Use of Hormonally Defined Serum-Free Medium to Grow Specific Cell Types in the Kidney: The Concept of Selective Media.- Use of Primary Kidney Cell Culture to Study Medically Related Problems of the Kidney.- References.- 7 Rat Hepatocytes in Culture: A Model for Studies of Growth Control during Experimental Chemical Hepatocarcinogenesis.- Control of DNA Synthesis in Cultures of Adult Rat Hepatocytes.- Factors Required for the Induction of DNA Synthesis.- Platelet-Derived Growth Factor as an Inhibitor of Normal Hepatocyte DNA Synthesis.- Stimulatory Activity for Normal Hepatocyte DNA Synthesis in the Serum from Cancer Patients and Carcinogen-Fed Rats.- Altered Response of Hepatocytes from Carcinogen-Treated Rats to PDGF.- Discussion.- References.- 8 Intratesticular Regulation: Evidence for Autocrine and Paracrine Control of Testicular Function.- Testicular Cell Culture.- Sertoli Cells.- Leydig Cell Culture.- Peritubular Myoid Cell Culture.- Culture of Germinal Cells.- Autocrine Regulation of Testicular Cells.- Sertoli Cells.- Leydig Cells.- Cell-Cell Interaction in the Testis.- Sertoli-Leydig Cell Interaction.- Sertoli-Myoid Cell Interaction.- Sertoli-Germ Cell Interaction.- Leydig-Myoid Cell Interaction.- Summary and Model of Intratesticular Regulation.- References.- 9 Attachment Factors in Cell Culture.- Collagen.- Biochemical and Biological Properties of Collagen.- The Use of Collagen in Cell Culture.- Effects of Collagen on Adhesion, Proliferation, and Differentiation of Cells in Culture.- Synthesis and Deposition of Collagen by Cells in Culture.- The Role of Collagen in Neoplastic Transformation.- Fibronectin.- Biochemical and Biological Properties of Fibronectin.- The Use of Fibronectin in Cell Culture.- Effects of Fironectin on Adhesion, Proliferation, and Differentiation of Cells in Culture.- Synthesis and Deposition of Fibronectin by Cells in Culture.- The Role of Fibronectin in Neoplastic Transformation.- Laminin.- Biochemical and Biological Properties of Laminin.- The Use of Laminin in Cell Culture.- Effects of Laminin on Adhesion, Proliferation, and Differentiation of Cells in Culture.- Synthesis and Deposition of Laminin by Cells in Culture.- Chondronectin.- Serum-Spreading Factor.- Biochemical and Biological Properties of Serum-Spreading Factor.- The Use of Serum-Spreading Factor in Cell Culture.- Effects of Serum-Spreading Factor on Adhesion, Proliferation, and Differentiation of Cells in Culture.- Epibolin.- Fetuin.- Extracellular Matrix in Cell Culture.- Future Trends: Studies of Cell Shape and Cell-Substratum Interactions.- References.- 10 Cell Culture Studies Using Extracts of Extracellular Matrix to Study Growth and Differentiation in Mammalian Cells.- Background on Extracellular Matrix.- Early Studies Indicating Relevance of Extracellular Matrix.- Chemical Composition of the Extracellular Matrix.- Known Functions of Matrix Proteins.- Cell Culture Methods Using Crude Extracts of Extracellular Matrix.- Extracellular Matrix.- Biomatrix.- Crude Extracts from Amniotic Membranes.- The Future.- References.