Checkpoint Controls and Cancer

Protocols for the Study of Checkpoint Regulatory Components.- Analysis of RB Action in DNA Damage Checkpoint Response.- Interaction Between the Retinoblastoma Protein and Protein Phosphatase 1 During the Cell Cycle.- Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site Analysis.- Functional Analysis of CDK Inhibitor p21WAF1.- Analysis of p21CDKN1A Recruitment to DNA Excision Repair Foci in the UV-Induced DNA Damage Response.- Quantitative Determination of p16 Gene Expression by RT-PCR.- Measuring Cyclin-Dependent Kinase Activity.- Determination of the Catalytic Activities of mTOR and Other Members of the Phosphoinositide-3-Kinase-Related Kinase Family.- CHK1 Kinase Activity Assay.- Assaying Cdc25 Phosphatase Activity.- Analyzing the Regulation and Function of ATM.- Use of siRNA to Study the Function of MDC1 in DNA Damage Responses.- Functional Analysis of APC-Cdh1.- Purification of the Mitotic Checkpoint Complex, an Inhibitor of the APC/C From HeLa Cells.- Analysis of the Spindle-Assembly Checkpoint in HeLa Cells.- Functional Analysis of the Spindle-Checkpoint Proteins Using an In Vitro Ubiquitination Assay.- Studying Consequences of Checkpoint Pathway Activation.- Analysis of Checkpoint Responses to Histone Deacetylase Inhibitors.- Biochemical Analysis of the Cell Cycle and Cell Cycle Checkpoints in Transiently Transfected Cells After Collection With Magnetic Beads.- Analysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA Substrates.- Analyzing Cell Cycle Checkpoints After Ionizing Radiation.- FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells.- Analysis of Cell Cycle by Flow Cytometry.- Analyzing Markers of Apoptosis In Vitro.- Analysis of Telomerase Activity and Telomere Function in Cancer.