Handbook of ELISPOT

Elispot Assay Setup and Data Analysis.- ELISPOT Assay.- Chemistry and Biology of the ELISPOT Assay.- Membranes and Membrane Plates Used in ELISPOT.- Standardization and Validation Issues of the ELISPOT Assay.- A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay.- Isolation of Subsets of Immune Cells.- Image Analysis and Data Management of ELISPOT Assay Results.- High Resolution as a Key Feature to Perform Accurate ELISPOT Measurements Using Zeiss KS ELISPOT Readers.- Elispot Assay in Animal Models.- Improving the Sensitivity of the ELISPOT Analyses of Antigen-Specific Cellular Immune Responses in Rhesus Macaques.- Feline Cytokine ELISPOT.- Measuring T-Cell Function in Animal Models of Tuberculosis by ELISPOT.- Interferon-? ELISPOT Assay for the Quantitative Measurement of Antigen-Specific Murine CD8+ T-Cells.- Elispot Assays in Human Studies.- Detection of Measles Virus-Specific Interferon-?-Secreting T-Cells by ELISPOT.- Epitope Mapping in Multiple Sclerosis Using the ELISPOT Assay.- Use of Interferon-? ELISPOT in Monitoring Immune Responses in Humans.- ELISPOT Determination of Interferon-? T-Cell Frequencies in Patients With Autoimmune Sensorineural Hearing Loss.- Multiplex and Modified Elispot Assay Formats.- Dual-Color ELISPOT Assay for Analyzing Cytokine Balance.- Simultaneous Detection of Multiple Cytokines in ELISPOT Assays.- Fluorospot Assay.- A Gel-Based Dual Antibody Capture and Detection Method for Assaying of Extracellular Cytokine Secretion.