Allergy Methods and Protocols

Understanding of the Molecular Mechanisms of Allergy.- T Cell — Primary Culture from Peripheral Blood.- Production of T-Cell Lines.- Production of Human T-Cell Clones.- Mapping of Human T-Cell Epitopes of Allergens.- Determining MHC Restriction of T-cell Responses.- Short-Term Culture of CD8 Cells and Intracellular Cytokine Staining.- Isolation, Flow Cytometric Analysis, and Suppression Assay of CD4+ CD25+ T-Regulatory Cells.- Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production.- Ultrasensitive ELISA for Measurement of Human Cytokine Responses in Primary Culture.- Quantification of Human Chemokine Production in TLR-Stimulated and Antigen-Specific Recall Responses.- Standardization of Allergen Extracts.- Immunoelectrophoresis for the Characterization of Allergen Extracts.- Conjugation of Haptens.- Monoclonal Antibodies.- Purification of Antibodies.- Collection of Air Samples to Quantify Exposure to Airborne Allergens.- Assay of Air Sample Eluates.- The Halogen Assay - A New Technique for Measuring Airborne Allergen.- Measurement of Specific IgG Anti-Fel d 1 Antibodies.- The Facilitated Antigen Binding (FAB) Assay - A Protocol to Measure Allergen-Specific Inhibitory Antibody Activity.- Microscopic Identification and Purity Determination of Pollen Grains.- Biopanning for the Characterization of Allergen Mimotopes.- Identification of Mast Cells and Mast Cell Subpopulations.- Purification and Characterization of Mast Cell Tryptase and Chymase from Human Tissues.- Experimental Activation of Mast Cells and Their Pharmacological Modulation.- In situ Hybridization.