Asthma

Asthma: Application of Cell and Molecular Biology Techniques to Unravel Causes and Pathophysiological Mechanisms.- Culture of Normal Human Airway Epithelial Cells and Measurement of Mucin Synthesis and Secretion.- Brush Biopsy and Culture of Airway Epithelial Cells: ?-Adrenergic Receptor System Function.- Isolation and Characterization of Human Airway Fibroblasts in Culture.- Flow Cytometric Analysis of Blood Monocytes and Alveolar Macrophages.- Expression of IL-10 and GM-CSF in Blood Monocytes and Alveolar Macrophages.- MAP Kinase Expression in Eosinophils.- Human Eosinophil Isolation and the Measurement of Apoptosis.- Adhesion of T-Cells to Airway Smooth Muscle Cells.- Proliferation of Circulating T-Cells.- Differential Display Analysis of Inflammatory Genes in Airway Epithelium.- Analysis of Transcription Factor Activation: NF?B as a Regulator of Inflammatory Genes in Epithelial Cells.- Transient Transgenic Approaches for Investigating the Role of GM-CSF in Pulmonary Inflammation and Immune Diseases.- Quantitative Analysis of RANTES mRNA in Endobronchial Biopsies Using Polymerase Chain Reaction.- Intracellular Cytokine Staining for Analysis by Flow Cytometry.- Localization of IL-4 and IL-5 mRNA in Bronchial Biopsies.- Immunohistochemical Analysis of Adhesion Molecules in Airway Biopsies.- Site-Directed Mutagenesis in the Investigation of ?-Adrenoreceptor Exosite.- Methods for Determining ?2-Adrenoreceptor Genotype.- Modulation of ?-Adrenoreceptor Expression in the Airways.- Chemical Mutational Analysis of Glucocorticoid Receptor.- Histone Acetylation and Deacetylation.- Genome-Wide Search for Asthma Genes.