Metastasis Research Protocols

Selection/Segregation Cell Populations for in Vitro and in Vivo Assays of Metastatic Behavior.- Cell Separations by Flow Cytometry.- Immunomagnetic Cell Separation.- Genetic Modification of Cell Lines to Enhance Their Metastatic Capability.- In Vitro Assays of Metastatic Behavior.- Cell Aggregation Assays.- Cell Migration and the Boyden Chamber.- Quantification of Cell Motility.- In Vitro Invasion Assay Using Matrigel®.- Membrane Invasion Culture System.- Collagen Invasion Assay.- Chick Heart Invasion Assay.- Adhesion of Tumor Cells to Matrices and Endothelium.- Assessment of Angiogenic Factors The Chick Chorioallantoic Membrane Assay.- Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis.- Galectin-3 Binding and Metastasis.- Animal Models of Metastasis.- Basic Principles for the Study of Metastasis Using Animal Models.- The Chick Embryo Metastasis Model.- GeneTransfection for Metastasis Research Using Animal Models.- Theoretical Considerations in Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice.- Xenograft Models in Immunodeficient Animals.- Xenograft Models in Immunodeficient Animals.- Syngeneic Murine Metastasis Models.- Transgenic Animal Models.- Clinically Accurate Orthotopic Mouse Models of Cancer.- Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo.- Green Fluorescent Protein for Metastasis Research.