Transcription Factor Protocols

Isolation of Target Gene Promoter/Enhancer Sequences by Whole Genome PCR Method.- In Vivo Footprinting Using UV Light and Ligation-Mediated PCR.- Identification of DNaseI Hypersensitive Sites Within Nuclei.- Analysis of In Vivo Methylation.- Detection of Transcription Factor Partners with a Yeast One Hybrid Screen.- Inverse PCR (IPCR) for Obtaining Promoter Sequence.- PCR-Directed Linker Scanning Mutagenesis.- Transfection Technologies.- The Use of Particle-Mediated Gene Transfer for the Study of Promoter Activity in Somatic Tissues.- Optimizing Electroporation Conditions for the Transformation of Mammalian Cells.- Calcium Phosphate Transfection of Mammalian Cultured Cells.- DEAE-Dextran Transfection of Mammalian Cultured Cells.- Liposome-Mediated Transfection of Mammalian Cells.- Assays for Transcriptional Activity Based on the Luciferase Reporter Gene.- Transient Transfection of Schneider Cells in the Study of Transcription Factors.- Triplex-Forming Oligonucleotides and Their Use in the Analysis of Gene Transcription.- Expression and Purification of Histidine-Tagged Transcription Factors.- Generation of Transcription Factors in Rabbit Reticulocyte Lysate Depleted of Endogenous DNA-Binding Protein.- Electrophoretic Mobility Shift Assays.- In Vitro Promoter Analysis Using Nuclear Extracts and G-Free Cassette Vectors.- In Vitro Transcription Using Competitor Oligonucleotides to Deplete Specific Transcription Factors.- Computer Software of Eukaryotic Promoter Analysis.