Immunological Diagnosis of Leukemias and Lymphomas

1. Introductory Lecture.- The immunological diagnosis of human leukemias and lymphomas: an overview.- 2. Clinical significance of T and B cell surface markers in lymphoid malignancies.- Surface markers define human lymphoid malignancies with differing prognoses.- Cell markers and the classification of acute leukemia.- Etiological, clinical and prognostic significance of the T-B immunological classification of primary acute lymphoid leukemias and Non-Hodgkin's lymphomas.- Surface receptors of human neoplastic lymphoreticular cells.- Non-Hodgkin lymphomas: cell populations and functional behavior.- 3. Leukemia-associated antigens versus cell differentation antigens in lymphatic and myeloic leukemias.- Membrane phenotyping: diagnosis, monitoring and classification of acute 'lymphoid' leukemias.- Cell differentiation antigens versus tumor-related antigens in childhood acute lymphoblastic leukemia (ALL). Clinical significance of leukemia markers.- Classification of leukemic cells with T- and O-ALL-specific antisera.- Membrane marker analysis of 'lymphoid' and myeloid blast crisis in Ph1 positive (chronic myeloid) leukemia.- Myeloid antigens and antigen densities in mice and men.- Expression of T and B cell markers and leukemia associated antigens on cell surface of human lymphocytic leukemias.- 4. Quantitative expression of surface markers on neoplastic lymphocytes.- Expression of FcIgG-receptor (FcIgG-r) sites on the surface of neoplastic lymphocytes.- Quantitation of T-antigenic sites and Ig-determinants on leukemic cells by microphotometric immunoradioautography. Proof of the clonal origin of thymus-derived lymphocytic leukemias.- Immunofluorometric analyses of membrane bound IgM in individual human lymphoid cells in cases of leukemia and malignant lymphoma.- 5.Characterization of leukemias and lymphomas by surface receptors and enzyme markers.- Hairy cell leukemia: simultaneous demonstration of autochthonous surface-Ig and monocytic functions of hairy cells.- Chronic lymphatic leukemia cells bear IgM receptors.- Acid phosphatase in acute lymphatic leukemia (ALL).- Lysosomal acid hydrolyses in isolated normal and chronic lymphocytic leukemia (CLL) T- and B-lymphocytes.- Prolymphocyte leukemia with T cell properties and tartrate resistant acid phosphatase.- Enzymecytochemical and immunocytological studies as a basis for the reevaluation of the histology of cutaneous B-cell and T-cell lymphomas.- Characterization of neoplastic mononuclear cells on tissue sections.- 6. Functional characteristics of neoplastic lymphocytes and/or of the resting lymphocyte population.- K cell activity of normal and chronic lymphocytic leukemia.- Comparative studies of the proportions of T- and B- and zero lymphocytes and the degree of blastic transformation of lymphocytes after stimulation by PHA and ConA in patients with Hodgkin's disease.- In vitro functions of lymphoid cells in untreated childhood acute lymphoblastic leukemia.- Difference in distribution and dynamics of cellmarkers on T-lymphoblasts compared to T-lymphocytes.- Immunological evaluation of 15 children with Non-Hodgkin lymphoma.- Studies on the immune status of children with acute lymphocytic leukemia (ALL).- Lymphocyte subpopulations in lymphatic tissues and blood of patients with malignant lymphomas.- 7. Establishment and characterization of malignant lymphoid cell lines.- Establishment and characterization of leukemic T-cell lines, B-cell lines, and null-cell line: a progress report on surface antigen study of fresh lymphatic leukemias in man.- Established cell lines as toolsin the study of human lymphoma and myeloma cell characteristics.- Characterization of 'T' and 'non-T' cell lines established from children with acute lymphoblastic leukemia and Non-Hodgkin lymphoma after leukemic transformation.- Establishment and characterization of a truly leukemic cell line.- Characterization of a human Hodgkin cell line and a lymphoblastic EBNA-negative cell line derived from a Non-Hodgkin lymphoma patient.- Autoradiographic detection of the Epstein-Barr virus (EBV) - associated early antigen (EA) in lymphoblastoid cell lines.- Lymphoid cell lines: in vitro cell markers in correlation to tumorigenicity in nude mice.- 8. Genetic markers define the mode of origin of lymphoid malignancies.- Glucose-6-phosphate dehydrogenase (G-6-PD) markers in Burkitt lymphoma and other malignancies.- The significance of chromosomal findings for the differentiation between lymphoma and lymphoblastoid cell lines.- Isoelectric focusing pattern of IgM and surface markers in Non-Hodgkin's lymphomas..- Idiotypic determinants and antiglobulins in multiple myeloma.- 9. Ia determinants, blood group antigens and other markers for characterization of leukemic cell types.- New aspects in the classification of leukemic cell types by la alloantigens and complement receptors.- Heterologous group specific antiserum against Iak determinants.- The autoantigen determinants I/i, Pr1-3 of normal and leukemic leucocytes.- Alterations of blood group receptors of erythrocytes in leukemia - Immunofluorescence studies.- Two antibodies in anti-human T-cell sera.- Isolation of antilymphocyte antibodies with a solid waterinsoluble not gel-like immunoadsorbent.- Cationic proteins of leucocyte lysosomes: marker antigens for the immunological diagnosis of acute leukemias.- 10.Summing up.- Author Index.- Correspondence List.