Biomedical Applications of Proteomics

Jean Charles Sanchez has been working since 1989 in the field of proteomics. He contributed in the development of two dimensional polyacrylamide gel electrophoresis and related procedures and their application in Biomaker discovery research. He obtained his Ph.D. in Biochemistry at the Buckingham University (UK) and in 1997 a Business Administration diploma (Officer School of Lausanne). Since 1995, he has been the head of the Biomedical Proteoomics Group at the BPRG at the Geneva University Hospital. He is a founder of the Swiss Proteomics Society, Swiss 2Dservice and Phenomics Sciences Consulting. The activities in his group cover the discovery of new disease markers and drug targets in multi factorial disorders including cancer, Alzheimer, CJD, stoke, obesity and type II diabetes. Garary L. Corthals has been involved in proteomics for over 10 years and has focused on integrating MS technologies for protein analysis in biomedical research. His current interests include development of technologies for rapid and sensitive protein analysis to discover new diagnostic markers for disease and to elucidate cellular processes that direct physiological behaviour. Other activities include the synthesis of new chemistries for accurate quantitation or proteins and the concurrent analysis of PTMs; as well as technologies for capturing high density protein profiles. He obtained his Ph.D. in Biology at Macquarie University (Australia) in the field of proteomics. Before arriving in Geneva he worked in the Department of Molecular Biotechnology (UW, Seattle) and directed the Proteomics Facility at the Garvan Institute of Medical Research in Sydney (Australia). He currently heads the MS Group at the BPRG at Geneva University Hospital and is co director of the Proteomics Facility of the Faculty of Medicine. Denis F. Hochstrasser is the director of the Clinical Pathology Department and Head of the Clinical Chemistry Laboratory of the Geneva University Hospital. He obtained his M.D. at Geneva Medical School, and did his internship and residency at UNC, Chapel Hill (USA). He is known for his pioneering development of what is now called proteomics. He initiated work on software development for proteomics data processing 15 years ago and has contributed to the development of the leading 2 D gel analysis software Melaine and related 2 D PAGE database. He was instru mental the creation of ExPASy, the fist life sciences devoted web server. Most recently he has been responsible for the Molecular Scanner, a technique that promises to enhance clinical proteomic analysis. He is a founder of the Swiss Institute for Bioinformatics and scientific founder of GeneProt & GeneBio.

List of Contributors. Abbreviations. Introduction (D. Hochstrasser, et al.). PART I: ASPECTS OF BIOMEDICAL RESEARCH. 1. Proteomics in Biomedicine A Tool, a Science or an Art? (M. Reymond). PART II: BLOOD VESSELS. 2. Antibody Based Vascular Targeting: Proteomic Techniques for the Identification and Quantitation of Membrane Proteins on Endothelial Cells (S. Scheurer, et al.). 3. Vasculature, Vascular Disease, and Atherosclerosis (E. Gianazza & I. Eberini). 4. Discovery of New Diagnostic Markers of Stroke (L. Allard, et al.). PART III: CANCER. 5. Unravelling Biological Pathways and the Identification of Clinical Markers and Targets in Renal Cancer (R. Banks & P. Selby). 6. Heat Shock Protein 27 in Cancer (C. Sarto, et al.). 7. Proteomic Approaches for Biomarker Discovery in Colorectal Cancer (R. Simpson & D. Dorow). 8. Clinical Proteomics: Ovarian Cancer (A. Alaiya). 9. Protein Expression Profiling Analysis in Hematopoietic Stem Cells: Phenotypic Characterization of Mesenchymal Stem Cells (J. Lopez, et al.). 10. Lymphoblastoid and Lymphoma Cells (R. Joubert Caron, et al.). PART IV: PHARMACO TOXICOLOGY. 11. Chemoresistance in Cancer Cells (J. Poland, et al.). 12. Diabetes Mellitus: Complex Molecular Alterations (G. Schmid & J. Sanchez). PART V: INFECTIOUS DISEASES. 13. Proteome Approach to Infectious Diseases: Acute phase Proteins and Antibody Profiles as Diagnostic Indicators in Human Plasma (L. Bini, et al.). 14. Proteomic Studies of Human Lymphocytes: New Insights Into HIV Lymphocyte Infection? (F. Vuadens, et al.). 15. Modifications of Host Cell Proteomes Induced by Herpes Virus Type 1 (A. Greco, et al.). 16. Francisella Tularensis (J. Stulik, et al.). PART VI: CENTRAL NERVOUS SYSTEM. 17. Proteomics in Clinical Neurosciences (P. Burkhard & J. Sanchez). 18. Human Cerebrospinal Fluid (P. Davidsson & M. Harrington). 19. Proteomic Applications for Molecular Assessment of Alzheimer s Disease (O. Carrette, et al.). PART VII: MASS SPECTROMETRY AND BIOINFORMATICS. 20. MALDI MS Imaging in Biomedical Research (M. Stoeckli & T. Farmer). 21. Protein Variations: Resources and Tools (Y. Yip, et al.). Subject Index.