Epidermal Cells

Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS).- Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal Microcultures.- Growth and Stratification of Epithelial Cells in Minimal Culture Conditions.- Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models.- Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice.- Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions.- Directed Differentiation of Human Embryonic Stem Cells to Epidermal Progenitors.- Expression and Analysis of Exogenous Proteins in Epidermal Cells.- Using siRNA Knockdown in HaCaT Cells to Study Transcriptional Control of Epidermal Proliferation Potential.- RNA Interference in Keratinocytes and an Organotypic Model of Human Epidermis.- Scanning for Transcription Factor Binding by a Variant EMSA.- Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes.- Gene Expression Profiling of Mouse Epidermal Keratinocytes.- Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection.- Comprehensive Transcriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents, and Cultured Keratinocytes Using DNA Microarray Chips.- Molecular Profiling of the Epidermis: A Proteomics Approach.- Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization.- Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts.- Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis.- Tetracycline-Regulated Gene Expression in Transgenic Mouse Epidermis.- A Versatile Murine 3D Organotypic Model to Evaluate Aspects of Wound Healing and Epidermal Organization.- Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic Epidermis.- Cytokine Release in Tissue-Engineered Epidermal Equivalents After Prolonged Mechanical Loading.- Three-Dimensional Human Tissue Models of Wounded Skin.- In Vivo Transplantation of Genetically Modified Mouse Embryonic Epidermis.- A Transplant Model for Human Epidermal Skin Regeneration.- Identification of Epithelial Stem Cells In Vivo and In Vitro Using Keratin 19 and BrdU.- Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spinal Cord Regeneration.- Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay.