Cytoskeleton Methods and Protocols

Live-Cell Imaging of the Cytoskeleton.- High Resolution Multimode Light Microscopy of Cell Migration: Long-Term Imaging and Analysis.- Imaging the Cytoskeleton in Live Xenopus laevis Embryos.- Live-Cell Imaging of the Cytoskeleton and Mitochondrial–Cytoskeletal Interactions in Budding Yeast.- Dynamics of Actin Filaments and Microtubules.- Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization.- Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts.- Fluorescence Microscopy Protocols for Investigating Cytoskeleton Components.- Quantitative Fluorescence Microscopy Techniques.- A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor Molecule.- Sample Preparation for Fluorescence Imaging of the Cytoskeleton in Fixed and Living Plant Roots.- Imaging of the Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells.- Electron Microscopy Protocols for Imaging the Cytoskeleton.- Imaging Cytoskeleton Components by Electron Microscopy.- Purification and Localization of Intraflagellar Transport Particles and Polypeptides.- High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophila Basal Bodies.- Strategies for Imaging Microtubules in Plant Cells.- Analysis of Cell and Organelle Motility.- A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity.- Melanosome Motility in Fish Retinal Pigment Epithelial Cells.- Analysis of Properties of Cilia Using Tetrahymena thermophila.- Application of Cell Traction Force Microscopy for Cell Biology Research.- 2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics.- Detergent-Extracted Models for the Study of Cilia or Flagella.- Geneticand Proteomics Approaches for Cytoskeleton Protein Function.- The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions.- Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila.- Proteomic Tools for the Analysis of Cytoskeleton Proteins.