The Biology of DNA Tumor Viruses

I. Introduction.- II. Structure of Viral DNA.- 1. Evidence for a Ring Structure of Polyoma Virus DNA.- 2. The Cyclic Helix and Cyclic Coil Forms of Polyoma Viral DNA.- 3. The Twisted Circular Form of Polyoma Viral DNA.- 4. A Dye-Buoyant-Density Method for the Detection and Isolation of Closed Circular Duplex DNA: The Closed Circular DNA in HeLa Cells.- III. Integration State of Viral DNA in Transformed Cells.- 1. Viral DNA in Polyoma- and SV40-Transformed Cell Lines.- 2. The Integrated State of Viral DNA in SV40-Transformed Cells.- IV. Detection of Virus-Specific (T) Antigen.- 1. A Specific Complement-Fixing Antigen Present in SV40 Tumor and Transformed Cells.- 2. Detection of Specific Antigen in SV40-Transformed Cells by Immunofluorescence.- 3. Synthesis of SV40 Tumor Antigen during Replication of Simian Papovavirus (SV40).- V. Activation of Infectious Tumor Virus SV40 in Transformed Cells.- 1. Studies on the Transfer of Subviral Infectivity from SV40-Induced Hamster Tumor Cells to Indicator Cells.- 2. Activation of Production of Infectious Tumor Virus SV40 in Heterokaryon Cultures.- 3. Production of SV40 Virus in Heterokaryons of Transformed and Susceptible Cells.- VI. Transcription of the Viral Genome In Infected and Transformed Cells.- 1. Virus-Specific RNA in Cells Productively Infected or Transformed by Polyoma Virus.- 2. Characterization of the Simian Virus 40-specific RNA in Virus-yielding and Transformed Cells.- 3. Regulation of Transcription of the SV40 DNA in Productively Infected and in Transformed Cells.- 4. The Transcription of the SV40 Genome in Productively Infected and Transformed Cells.- 5. Strand Orientation of Simian Virus 40 Transcription in Productively Infected Cells.- 6. A Map of Simian Virus 40 Transcription Sites Expressed in Productively Infected Cells.- 7. Presence of Cell and Virus Specific Sequences in the Same Molecules of Nuclear RNA from Virus Transformed Cells.- VII. Temperature-Sensitive Mutant Studies of Cell Transformation in Polyoma Virus.- 1. Induction of Cellular DNA Synthesis by Polyoma Virus.- 2. Abortive Transformation by Polyoma Virus.- 3. Cell-Transforming Ability of a Temperature-Sensitive Mutant of Polyoma Virus.- 4. Abortive Transformation by the Tsa Mutant of Polyoma Virus.- 5. Temperature-Dependent Properties of Cells Transformed by a Thermosensitive Mutant of Polyoma Virus.- VIII. Further Problems in the Biology of Small DNA Viruses.- 1. Growth Control in Cultured Cells: Selection of Sublines with Increased Sensitivity to Contact Inhibition and Decreased Tumor-Producing Ability.- 2. Mutation Causing Temperature-Sensitive Expression of Cell Transformation by a Tumor Virus.- Author Index.