Bone Marrow Immunohistochemistry

Despite advances in flow cytometry and molecular methods, immunohistochemistry (IHC) remains an important, useful, and cost-effective component of the diagnostic evaluation of most bone marrow biopsies. In fact, the use of IHC promises to grow as the menu of primary antibodies that can be applied to bone marrow biopsy grows at an exponential rate. Yet, to date, there has been no single resource that addresses the utility of individual IHC markers, their selection, and their appropriate application and interpretation in specific disease processes involving the bone marrow. By providing a comprehensive overview of the use of IHC markers in bone marrow biopsies, this book fills this critical gap in the literature. It provides a rich collection of colour illustrations that demonstrate the diagnostic features of antibodies applicable to bone marrow tissue.

Each of the antibodies illustrated in the book includes a systematic description that details an expected reaction profile in both normal bone marrow cells and neoplastic cells, as well as authoritative advice about avoiding potential diagnostic pitfalls and technical problems related to the antibodies and tissue processing.

A completely practical volume for pathologists and pathology residents alike, this book presents a methodological approach. Included are critical reviews of most of the IHC tests in use today, as well as summaries of expected reactivities in normal and diseased bone marrow. Also included is information about those markers less commonly used, in order to provide essential support for the correct interpretation of results in specific diagnostic settings.

Pathology residents will find the volume particularly useful as a quick and efficient tool for learning about expected patterns of staining in a variety of conditions and the limitations of each technique.