PCR Methods Manual

Scientists from universities, industry, and government agencies, mostly in the US but also in Europe explain the methods for using the polyermase chain reaction technique in mRNA quantitation, diagnosis, gene discovery, genomic analysis, and expression profiling. They set out the key concepts such as thermostable DNA polymerases and optimization gu

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PCR is considered the most powerful technique used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 1980s, PCR has blossomed into a method that enables everything from the ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. This work examines the developments in this field. It discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the bioinformatics boom.