Giant Vesicles

Pier Luigi Luisi and Peter Walde, both of Institut fur Polymere, ETH-Zurich, Switzerland

Contributors. Preface. Part One: INTRODUCTION. 1 Why Giant Vesicles? (Pier Luigi Luisi). 2 Giant Vesicles: a Historical Introduction (Danilo D. Lasic). Part Two: PREPARATION METHODS. 3 Liposome Electroformation (Miglena I. Angelova). 4 Formation of Giant Vesicles from Different Kinds of Lipids Using the Electroformation Method (Aline Fischer, Pier Luigi Luisi, Thomas Oberhober and Peter Walde). 5 Observation of a Variety of Giant Vesicles under an Optical (Ken-ichirou Akashi, Kazuhiko Kinosita, Jr., Hidetake Miyata and Hiroyasu Itoh). Part Three: BASIC THEORETICAL ASPECTS. 6 Bending Elasticity of Fluid Membranes (Wolfgang Helfrich). 7 Giant Vesicles: a Theoretical Perspective (Udo Seifert). 8 Free Energy of a Fluctuating Vesicle. Influence of the Fluctuations on the Laplace Law (Isak Bivas). Part Four: PHYSICAL PROPERTIES. 9 Use of Micropipet Manipulation Techniques to Measure the Properties of Giant Lipid Vesicles (David Needham and Doncho Zhelev). 10 Fluctuating Vesicle Shapes (Hans-Gunther Dobereiner). 11 Oblate-Prolate Transition of Ellipsoidal Magnetoliposomes: Experiments showing an Anisotropic Spontaneous Curvature (Olivier Sandre, Christine Menager, Jerome Prost, Valerie Cabuil, Jean-Claude Bacri and Andrejs Cebers). 12 Micromanipulation of Tubular Vesicles (Liyu Xu and Hans-Gunther Dobereiner). 13 Electromechanical Properties of Model Membranes and Giant Vesicle Deformations (Philippe Meleard, Claire Gerbeaud Tanja Pott and Marin D. Mitov). 14 Mechanical Properties of Lipid Bilayers Containing Grafted Lipids (Isak Bivas, Victoria Vitkova, Marin D. Mitov, Mathias Winterhalter, Rossitsa G. Alargova, Philippe Meleard and Pierre Bothorel). 15 Motion of Particles Attached to Giant Vesicles: Falling Ball Viscosimetry and Elasticity Measurements on Lipid Membranes (Rumiana Djmova, Christian Dietrich and Bernard Pouligny). 16 Control of Fusion Between Giant Vesicles (Sek Wen Hui, Meghan Perkins and Parthapratim Chandaroy). 17 Membrane Roughness and Dispersive Phase as Effects of Higher-order Bending in Fluid Membranes (Beate Klosgen and Wolfgang Helfrich). 18 Swelling and Separation of DOPC Multilayer Systems (Johannes Thimmel, Beate Kliisgen, Wolfgang Helfrich and Gert Rapp). 19 Dynamic Aspects of Fatty Acid Vesicles: pH-induced Vesicle-Micelle Transition and Dilution-induced Formation of Giant Vesicles (Ayako Goto, Akihiro Suzuki, Hisashi Yoshioka, Rensuke Goto, Toyoko Imae, Keiji Yamazaki and Peter Walde). Part Five: CHEMICAL AND BIOLOGICAL ASPECTS. 20 Giant Liposomes as Model Biomembranes for Roles of Lipids in Cellular Signalling (Paavo K. J. Kinnunen, Juha M. Holopainen and Miglena I. Angelova). 21 Microinjection of Macromolecules in Giant Vesicles Prepared by Electroformation (Thomas Oberholzer and Aline Fischer). 22 Enzymatic Reactions in Giant Vesicles (Peter Walde). 23 Giant Phospholipid Vesicles Entrapping Giant DNA (Shin-ichirou Nomura and Kcnichi Yoshikawa). 24 Cell Deformation Mechanisms Studied with Actin-containing Giant Vesicles, a Cell-mimicking System (Hidetake Miyata, Kazuo Ohki, Gerard Marriott, Shuji Nishiyama, Ken-ichirou Akashi and Kazuhiko Kinosita, Jr.). 25 Light-Induced Shape Transitions of Giant Vesicles (Peter G. Petrov and Hans-Gunther Dobereiner). 26 Changes in the Morphology of Giant Vesicles Under Various Physico-chemical Stresses (Marie-Alice Guedeau-Boudeville, Anne-Laure Bernard, Jean-Claude Bradley, Alok Singh and Ludovic Jullien). 27 Magnification of Shape Fluctuations of Active Giant Unilamellar Vesicles (Jean-Baptiste Manneville, Patricia Bassereau, Daniel Levy and Jacques Prost). 28 Entrapment of Proteins in Soybean Phosphatidylcholine Vesicles (Masanao Imai, Kazuhito Nagayama, Hikaru Tanaka, Nobukazu Osaki and Toshifusa Doi). 29 Study on Stress-mediated Behavior and Preparation of Giant Vesicles (Toshinori Shimanouchi, Hiroshi Umakoshi and Ryoichi Kuboi). 30 Molecular Organization on Giant Unilamellar Vesicles (Susanne Gangl, Susanne Stark, Peter Mayrhofer, Dominik Runzler, Caroline Thurner, Arno Lukas, Bernd Mayer and Gottfried Kohler). 31 Membrane Properties of Archaeal Phospholipids: Effect of Macrocyclization (Olivier Dannenmuller, Yoichi Nakatani, Guy Ourisson, Kenji Arakawa, Tadashi Eguchi, Katsumi Kakinuma, Sylvie Blanc and Anne-Marie Albrecht). Cumulative Author Index. Cumulative Title Index. Index.