Base Excision Repair Pathway

Simultaneous Short and Long Patch Base Excision Repair (BER) Assay in Live Mammalian Cells.- In Vitro Assay to Measure APE1 Enzymatic Activity on Ribose Monophosphate Abasic Site.- Highly Sensitive Radioactivity-Based DNA 3’-Phosphatase Activity Assay for Polynucleotide Kinase 3’-Phosphatase.- Generation of Recombinant Nucleosomes Containing Site-Specific DNA Damage.- A DNA Cleavage Assay Using Synthetic Oligonucleotide Containing a Single Site-Directed Lesion for In Vitro Base Excision Repair Study.- In Vitro Reconstitutive Base Excision Repair (BER) Assay.- Detection of Oxidatively Modified Base Lesion(s) in Defined DNA Sequences by FLARE Quantitative PCR.- Isolation and Immunodetection of Enzymatic DNA-Protein Crosslinks by RADAR Assay.- Slot Blot Assay for Detection of R Loops.- Assays with Patient-Derived Organoids to Evaluate the Impact of Microbial Infection on the Base Excision Repair (BER) Enzymes.- Characterizing the Repair of DNA Double-Strand Breaks: A Review of Surrogate Plasmid-Based Reporter Methods.- Interactome Profiling of DNA Damage Response (DDR) Mediators with Immunoprecipitation-Mass Spectrometry.- Using Affinity Pulldown Assays to Study Protein-Protein Interactions of Human NEIL1 Glycosylase and the Checkpoint Protein RAD9-RAD1-HUS1 (9-1-1) Complex.- Tandem Affinity Purification and Mass-Spectrometric Analysis of FACT and Associated Proteins.- Analysis of Copy Number Variation of DNA Repair/Damage Response Genes in Tumor Tissues.- Genome-Wide Binding Analysis of DNA Repair Protein APE1 in Tumor Cells by ChIP-Seq.- Tumorsphere Formation Assay: A Cancer Stem-Like Cell Characterization in Pediatric Brain Cancer Medulloblastoma.