Phase-Separated Biomolecular Condensates

Calculating Binodals and Interfacial Tension of Phase-Separated Condensates from Molecular Simulations, with Finite-Size Corrections.- Field-Theoretic Simulation Method to Study the Liquid-Liquid Phase Separation of Polymers.- Numerical Techniques for Applications of Analytical Theories to Sequence-Dependent Phase Separations of Intrinsically Disordered Proteins.- An Introduction to the Stickers-and-Spacers Framework as Applied to Biomolecular Condensates.- Multi-Scale Modeling of Protein-RNA Condensation In and Out of Equilibrium.- Fluorescence Lifetime Imaging Microscopy of Biomolecular Condensates.- Single-Molecule Fluorescence Methods to Study Protein-RNA Interactions Underlying Biomolecular Condensates.- Fluorescence Correlation Spectroscopy and Phase Separation.- Measurement of Protein and Nucleic Acid Diffusion Coefficients within Biomolecular Condensates Using In-Droplet Fluorescence Correlation Spectroscopy.- Single-Molecule Imaging of the Phase Separation-Modulated DNA Compaction to Study Transcriptional Repression.- Phase Separation-Based Biochemical Assays for Biomolecular Interactions.- Determining Thermodynamic and Material Properties of Biomolecular Condensates by Confocal Microscopy and Optical Tweezers.- A High-Throughput Method to Profile Protein Liquid-Liquid Phase Separation.- Phase Separation of Rubisco by the Folded SSUL Domains of CcmM in Beta-Carboxysome Biogenesis.- Cryo-Electron Tomography of Reconstituted Biomolecular Condensate.- Sedimentation Assays to Assess the Impact of Post-Translational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells.- Synthetic Organelles for Multiple mRNA Selective Genetic Code Expansion in Eukaryotes.- Single Molecule Tracking of RNA Polymerase In and Out of Condensates in Live Bacterial Cells.- An Optogenetic Toolkit for the Control of Phase Separation in Living Cells.- Assessing the Phase Separation Propensity of Proteins in Living Cells Through Optodroplet Formation.- Mass Balance Imaging: A Phase Portrait Analysis for Characterizing Growth Kinetics of Biomolecular Condensates.- Characterizing Properties of Biomolecular Condensates Below the Diffraction Limit In Vivo.