Prokaryotic Gene Regulation

Chromosome Conformation Capture in Bacteria and Archaea.- Micrococcal Nuclease Digestion Assays for the Analysis of Chromosome Structure in Archaea.- Detecting DNA Methylations in the Hyperthermoacidophilic Crenarchaeon Sulfolobus acidocaldarius using SMRT-Sequencing.- ProD: A Tool for Predictive Design of Tailored Promoters in Escherichia coli.- Computational Study on the Dynamics of Mycobacterium Tuberculosis RNA Polymerase Assembly.- In Vitro Transcription Assay for Archaea Belonging to Sulfolobales.- Prediction of DNA-Binding Transcription Factors in Bacteria and Archaea Genomes.- In Vivo Screening Method for the Identification and Characterization of Prokaryotic, Metabolite-Responsive Transcription Factors.- Application of Special Electron Microscopy Techniques to the Study of DNA-Protein Complexes in E. coli Cells.- High-Speed Atomic Force Microscopy Visualization of Protein-DNA Interactions using DNA Origami Frames.- Separation and Characterization of Protein-DNA Complexes by EMSA and In-Gel Footprinting.- Chemical Protection and Premodification Binding Interference for the Identification of Phosphate and Base-Specific Contacts in Protein-DNA Complexes.- Analysis of Protein-DNA Interactions using Isothermal Titration Calorimetry: Successes and Failures.- Determination of RNA Structure with In Vitro SHAPE-Experiments.- Microscale Thermophoresis to Study RNA-RNA Binding Affinity.- Toeprint Assays for Detecting RNA Structure and Protein-RNA Interactions.