DNA Damage Responses

Robust Computational Approaches to Defining Insights on the Interface of DNA Repair with Replication and Transcription in Cancer.- A Whole Genome CRISPR/Cas9 Screening Approach for Identifying Genes Encoding DNA End-Processing Proteins.- Immunoaffinity Purification of Epitope-Tagged DNA Repair Complexes from Human Cells.- Universally Accessible Structural Data on Macromolecular Conformation, Assembly, and Dynamics by Small Angle X-Ray Scattering for DNA Repair Insights.- Assessing DNA damage responses using B lymphocyte cultures.- Studying single stranded DNA gaps a­­t replication intermediates by electron microscopy.- Approaches to monitor termination of DNA replication using Xenopus egg extracts.- Use of high-performance liquid chromatography coupled to mass spectrometry to quantify modified nucleosides.- Targeted Formation of 8-oxoguanine in Telomeres.- Qualitative and Quantitative Analysis of DNA-Cytidine Deaminase Activity.- Characterization of DNA-PK-bound end fragments using GLASS-ChIP.- Monitoring Nuclease Activity by X-ray Scattering Interferometry using Gold Nanoparticle Conjugated DNA.- In Vitro Reconstitution of BRCA1-BARD1/RAD51 Mediated Homologous DNA Pairing.- Purification of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) fromHeLa cells.- Purification and characterization of human DNA ligase III complexes after expression in insect cells.- Generation of monoubiquitin and K63-linked polyubiquitin chains for protein interaction studies.