Plant Circular RNAs -

Plant Circular RNAs

Methods and Protocols

Luis María Vaschetto (Herausgeber)

Buch | Softcover
196 Seiten
2022 | 1st ed. 2021
Springer-Verlag New York Inc.
978-1-0716-1647-5 (ISBN)
128,39 inkl. MwSt
​This volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants. The chapters in this book summarize the concepts and techniques for prediction/identification, validation, and analysis of plant circRNAs and their regulatory targets. Some of the topics covered are procedures for circRNA identification and characterization; strategies for circRNA generation; next generation sequencing (NGS) technologies and circRNAs’ research; and bioinformatics tools and pipelines for the analysis of circRNA-miRNA-mRNA regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and practical, Plant Circular RNAs: Methods and Protocols is an important reference for any researcher looking to explore and apply the latest developments made in this field. 

Identification and Characterization of Plant Circular RNAs.- Experimental Strategies for Studying the Function of Plant circRNAs.- Generation of Transgenic Rice Expressing circRNA and its Functional Characterization.- Identification of Circular RNAs by Multiple Displacement Amplification and their Involvement in Plant Development.- Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing.- Identification and Functional Characterization of Viroid Circular RNAs.- Circular RNA Databases.- NGS Methodologies and Computational Algorithms for the Prediction and Analysis of Plant Circular RNAs.- Computational Analysis of Transposable Elements and circRNAs in Plants.- Constructing circRNA-miRNA-mRNA Regulatory Networks by using GreenCircRNA Database.- Methods for Predicting circRNA-miRNA-mRNA Regulatory Networks: GreenCircRNA and PlantCircNet Databases as Study Cases.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 2362
Zusatzinfo 43 Illustrations, black and white; X, 196 p. 43 illus.
Verlagsort New York, NY
Sprache englisch
Maße 178 x 254 mm
Themenwelt Mathematik / Informatik Informatik Theorie / Studium
Informatik Weitere Themen Bioinformatik
Naturwissenschaften Biologie Botanik
Naturwissenschaften Biologie Genetik / Molekularbiologie
Schlagworte circular RNAs (circRNAs) • comparative genomics • Quantitative real-time PCR (qRT-PCR) • RNA immunoprecipitation • Sequencing Data
ISBN-10 1-0716-1647-1 / 1071616471
ISBN-13 978-1-0716-1647-5 / 9781071616475
Zustand Neuware
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