Molecular Biology LabFax - T. A. Brown

Molecular Biology LabFax

Gene Analysis

(Autor)

Buch | Hardcover
255 Seiten
1998 | 2nd edition
Academic Press Inc (Verlag)
978-0-12-136110-5 (ISBN)
102,25 inkl. MwSt
Divided into two parts: Recombinant DNA, and Gene Analysis, this book presents a collection of the various methods available in molecular biology. It provides information on nucleic acid blotting and hybridization, DNA sequencing, PCR, labelingnucleic acids, electrophoresis, centrifugation, chemicals and reagents, safety, and more.
In the eight years since the original publication of Molecular Biology Labfax, there has been a vast proliferation of molecular biology techniques. The Second Edition has been divided into two parts: Recombinant DNA, and Gene Analysis. Together they comprise a comprehensive collection of the most up-to-date methods available in molecular biology. This second volume of the two-part Second Edition provides key information on nucleic acid blotting and hybridization, DNA sequencing, PCR, labelingnucleic acids, electrophoresis, centrifugation, chemicals and reagents, and safety. Molecular Biology Labfax will be essential for scientists of all disciplines within the life sciences who use molecular biological techniques.

Abbreviations.
Nucleic Acid Blotting and Hybridization:
Membranes for Nucleic Acid Blotting.
Factors Influencing Hybridization.
Synthetic Oligonucleotide Probes.
Reagents and Conditions for Nucleic Acid Hybridization.
DNA Sequencing:
Chain Termination Sequencing.
Chemical Degradation Sequencing.
Denaturing Polyacrylamide Gel Electrophoresis.
Troubleshooting.
DNA Databases and Software for Sequence Analysis.
Polymerase Chain Reaction:
Amplification Methodology.
Cloning PCR Products.
Sequencing PCR Products.
Troubleshooting.
Labeling Nucleic Acids:
Radiochemistry.
Labeling Procedures.
Detection of Radioactivity.
Protection from Radiochemical Hazards.
Non-Radioactive Labeling.
Agarose Gel Electrophoresis:
Agarose Gels.
Electrophoresis Systems.
DNA and RNA Markers for Agarose Gels.
Centrifugation:
Calculation of the Relative Centrifugal Force.
Density Gradient Centrifugation.
Care of Rotors.
Chemicals and Reagents:
Details of Individual Compounds.
Safety:
Protection from Chemical Hazards.
Protection from Microbiological Hazards.

Erscheint lt. Verlag 6.7.1998
Reihe/Serie Labfax
Verlagsort San Diego
Sprache englisch
Maße 171 x 248 mm
Gewicht 720 g
Themenwelt Informatik Weitere Themen Bioinformatik
Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Zellbiologie
ISBN-10 0-12-136110-1 / 0121361101
ISBN-13 978-0-12-136110-5 / 9780121361105
Zustand Neuware
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