Systems Chemical Biology

1. PREDECT protocols for complex 2D/3D cultures.- 2. Phenotypic Screening using Mouse and Human Stem Cell-based Models of Neuroinflammation and Gene Expression Analysis to Study Drug Responses.- 3. Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer.- 4. Target Engagement of Small Molecules – Thermal Profiling Approaches on Different Levels.- 5. Visualizing Bio-active Small Molecules by Alkyne-tagging and Slit-scanning Raman Microscopy.- 6. The Cell Painting Assay as a screening tool for the discovery of bioactivities in new chemical matter.- 7. Proteomic Profiling for Target Identification of Biologically Active Small Molecules using 2-D DIGE.- 8. Examining Cellular Responses to Kinase Drug Inhibition through Phosphoproteome Mapping of Substrates.- 9. CRISPR/Cas9-based Chemogenomic Profiling in Mammalian Cells.- 10. Exome Sequencing of Drug-resistant Clones for Target Identification.- 11. The use of large-scale chemically-induced transcriptomedata acquired from LINCS to study small molecules.- 12. Integrated Analysis of Drug Sensitivity and Selectivity to Predict Synergistic Drug Combinations and Target Co-addictions in Cancer.- 13. Predicting Drug Interactions from Chemogenomics using INDIGO.- 14. Computational Analyses Connect Small-Molecule Sensitivity to Cellular Features Using Large Panels of Cancer Cell Lines.- 15. Web-based tools for Polypharmacology Prediction.- 16. In silico Target Prediction for Small Molecules.