DNA Topoisomerase Protocols

to DNA Topoisomerases.- Resolution of DNA Molecules by One-Dimensional Agarose-Gel Electrophoresis.- Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomers.- Analysis of Altered DNA Structures.- Purification of Supercoiled Plasmid DNA.- Purification and Use of DNA Minicircles with Different Linking Numbers.- Isolation of Kinetoplast DNA.- Isolation of Knotted DNA from Coliphage P4.- Analysis of DNA Knots and Catenanes by Agarose-Gel Electrophoresis.- Sedimentation Analysis of Bacterial Nucleoid Structure.- Coating DNA with RecA Protein to Distinguish DNA Path by Electron Microscopy.- Methods for Analyzing DNA Bending.- Formation of Extrachromosomal DNA Rings in Saccharomyces cerevisiae Using Site-Specific Recombination.- Overexpression and Purification of Bacterial DNA Gyrase.- Overexpression and Purification of Bacterial DNA Topoisomerase I.- Overexpression and Purification of Escherichia coliDNA Topoisomerase III.- Overexpression and Purification of Bacterial Topoisomerase IV.- Purification of the Bacteriophage T4 Type II DNA Topoisomerase.- Overexpression and Purification of DNA Topoisomerase I from Yeast.- Overexpression and Purification of Saccharomyces cerevisiae DNA Topoisomerase II from Yeast.- Purification of DNA Topoisomerase II from Drosophila melanogaster.- Purification of DNA Topoisomerase I from Human Placenta.- Purification of Baculovirus-Expressed Human DNA Topoisomerase I.- Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatography.- Metabolic Labeling, Immunoprecipitation, and Two-Dimensional Tryptic Phosphopeptide Mapping of Human Topoisomerase II.- Immunoblot Analysis and Band Depletion Assays.- Visualization of DNA Topoisomerases by Electron Microscopy.- Appendix: Compendium of DNA Topoisomerase Sequences.