Gene Cloning and DNA Analysis

Professor Terry Brown, Professor of Biomolecular Archaeology, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, UK Professor Brown became fascinated with the natural world at a young age. He began his research career studying the effects of metal pollution on microorganisms and the tolerance that some plants display to high concentrations of toxic metals. He then became excited by DNA and worked on mitochondrial genes in fungi in order to learn the new (in those days) techniques for gene cloning and DNA sequencing. He contributed to the discovery of mitochondrial Group 1 introns and to work that described the base-paired structure of these introns. He then became interested in ancient DNA and was one of the first people internationally to carry out DNA extractions with bones and preserved plant remains. This work has required close collaboration with archaeologists, both in Manchester and elsewhere, and led to current interests in the origins of agriculture, genetic profiling of archaeological skeletons, and the evolution of disease. Professor Brown was appointed Professor of Biomolecular Archaeology at UMIST in 2000 and was Head of Biomolecular Sciences at UMIST from 2002–2004, the two years leading up to the merger of UMIST and the Victoria University of Manchester and the successful creation of the new Faculty of Life Sciences. He then acted as Associate Dean for Communication and External Relations in FLS until 2006, and more recently as Head of the Molecular Systems Section. Professor Brown has written a number of undergraduate textbooks including Gene Cloning and DNA Analysis: An Introduction (6th edition, Wiley-Blackwell, 2010) and Genomes (3rd edition, Garland Science, 2006). As well as new editions of these books, he has written a new introductory genetics textbook published by Garland in 2011 and, with Keri Brown, a book on Biomolecular Archaeology published by Wiley-Blackwell, also in 2011.

Part I The Basic Principles of Gene Cloning and DNA Analysis 1 1 Why Gene Cloning and DNA Analysis are Important 3 2 Vectors for Gene Cloning: Plasmids and Bacteriophages 13 3 Purification of DNA from Living Cells 25 4 Manipulation of Purified DNA 47 5 Introduction of DNA into Living Cells 75 6 Cloning Vectors for Escherichia coli 93 7 Cloning Vectors for Eukaryotes 111 8 How to Obtain a Clone of a Specific Gene 135 9 The Polymerase Chain Reaction 157 Part II The Applications of Gene Cloning and DNA Analysis in Research 173 10 Sequencing Genes and Genomes 175 11 Studying Gene Expression and Function 201 12 Studying Genomes 225 Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology 245 13 Production of Protein from Cloned Genes 247 14 Gene Cloning and DNA Analysis in Medicine 269 15 Gene Cloning and DNA Analysis in Agriculture 291 16 Gene Cloning and DNA Analysis in Forensic Science and Archaeology 311 Glossary 329 Index 345