DNA Replication

A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells.- Microscopy Techniques to Examine DNA Replication in Fission Yeast.- High-Resolution Analysis of Mammalian DNA Replication Units.- Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing.- Measuring DNA Content by Flow Cytometry in Fission Yeast.- Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast.- Edu Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast.- Determination Of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection.- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo.- Detection And Sequencing Of Okazaki Fragments In S. Cerevisiae.- Chip-Seq To Analyze The Binding Of Replication Proteins To Chromatin.- Chromatin Immunoprecipitation To Detect DNA Replication And Repair Factors.- Molecular Genetic Methods To Study DNA Replication Protein Function In Haloferax Volcanii, A Model Archaeal Organism.- Single-Molecule Observation Of Prokaryotic DNA Replication.- Analysing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast.- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis.- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.