Chromatin Protocols

Beads-on-a-String on a Bead: Reconstitution and Analysis of Chromatin on a Solid Support.- Preparation and Analysis of Positioned Mononucleosomes.- Chromatin Imaging with Time-Lapse Atomic Force Microscopy.- Isolation of Specific Genomic Regions and Identification of Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using CRISPR.- Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin.- Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes.- Histone Deacetylase Activity Assay.- In Vitro Histone Demethylase Assays.- Integrated DNA Methylation and Chromatin Structural Analysis at Single-Molecule Resolution.- Determination of DNA Methylation Levels Using Illumina HumanMethylation450 BeadChips.- Investigation of Genomic Methylation Status Using Methylation Specific and Bisulfite Sequencing Polymerase Chain Reaction.- In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination.- Immunostaining Analysis of Tissue Cultured Cells and Tissue Sections Using Phospho-Histone H3 (Serine 10) Antibody.- Identification and Characterization of Nonhistone Chromatin Proteins: Human Positive Coactivator 4 as a Candidate.- Methods to Study Transcription-Coupled Repair in Chromatin.- Analysis of DNA Replication Associated Chromatin Decondensation: In Vivo Assay for Understanding Chromatin Remodeling Mechanisms of Selected Proteins.- Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length.- Detecting ATM Dependent Chromatin Modification in DNA Damage Response.- Imaging Local Deposition of Newly Synthesized Histones in UVC-Damaged Chromatin.- In Vitro Replication Assay with Mammalian Cell Extracts.- Fluorescent In Situ Hybridization on Comets: FISH Comet.- Methods to Study Histone Chaperone Function in Nucleosome Assembly and Chromatin Transcription.- Preparation of Mononucleosomal Templates for Analysis of Transcription with RNA Polymerase Using spFRET.- Transcriptome-Wide Identification of In Vivo Interactions Between RNAs and RNA-Binding Proteins by RIP and PAR-CLIP Assays.- Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications In Vivo.- ChIP on Chip and ChIP-Seq Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications.- ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays.