DNA Sequencing Protocols

Introduction to DNA Sequencing. The Universal Primers and the Shotgun DNA Sequencing Method. M13 Sequencing. Primer Design and Primer-Directed Sequencing. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction. Solid Phase Fluorescent Sequencing of the CFTR Gene. Shotgun DNA Sequencing. Cycle Sequencing. Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers. Automated Fluorescent DNA Sequencing on the ABI PRISM 377. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer. Fluorescent Sequencing Protocols for the ALF. Fluorescent Sequencing for Heterozygote Mutation Detection. Sequence Databases and the Internet. DNA Sequencing by Capillary Array Electrophoresis. Index.

"DNA Sequencing Protocols covers the commonly used protocols in a clearly written manner and is thorough in description. This book is highly recommended for all libraries serving researchers involved in any aspect of DNA sequencing." - E-STREAMS