DNA Methylation Protocols
Humana Press Inc. (Verlag)
978-0-89603-618-5 (ISBN)
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"DNA Methylation Protocols" offer a set of readily reproducible protocols of the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation and the isolation and measurement of demethylases and related proteins.
Overview. Nearest-Neighbor Analysis. Measurement of Genome-Wide DNA Cytosine-5 Methylation by Reversed-Phase High-Pressure Liquid Chromatography. Methylation Analysis by Chemical DNA Sequencing. Methylation-Sensitive Restriction Fingerprinting. Restriction Landmark Genome Scanning. Combined Bisulfite Restriction Analysis (COBRA). Differential Methylation Hybridization Using CpG Island Arrays. Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences. Isolation of CpG Islands Using a Methyl-CpG Binding Column. Purification of MeCP2-Containing Deacetylase from Xenopus laevis. DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method. Measuring DNA Demethylase Activity In Vitro. Extracting DNA Demethylase Activity from Mammalian Cells. Index.
| Erscheint lt. Verlag | 1.3.2002 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; v. 200 |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 152 x 229 mm |
| Gewicht | 446 g |
| Einbandart | gebunden |
| Themenwelt | Informatik ► Weitere Themen ► Bioinformatik |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| ISBN-10 | 0-89603-618-9 / 0896036189 |
| ISBN-13 | 978-0-89603-618-5 / 9780896036185 |
| Zustand | Neuware |
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