DNA-Protein Interactions

Expression Systems ; Gel electrophoresis and bending in cisplatin-modified linear DNA ; Use of DNA microcircles in protein-DNA binding studies ; Use of topology to measure protein-induced DNA bend and unwinding angles ; Bandshift, gel retardation, or electrophoretic mobility shift assays ; Practical aspects of Fluorescence Resonance Energy Transfer (FRET) and its applications in nucleic acid biochemistry ; Determination of DNA-ligand interactions by fluorescence correlation spectroscopy ; DNA wrapping in E. Coli RNA polymerase open promotor complexes revealed by scanning force microscopy ; Microcalorimetry of protein-DNA interactions ; DNA-protein cross-linking by formaldehyde: a probe for RNAP-open promoter complex structure ; Solid-phase DNAse I footprinting ; Hydroxyl radical footprinting ; Radiolytic cleavage of DNA: Mapping of the Protein Interaction Sites ; UV - Laser photoreactivity of nucleoprotein complexes in vitro ; In vivo UV-laser footprinting ; Digitization and quantitative analysis of footprinting gels ; Mapping histone positions in chromatin by protein-directed DNA cross-linking and cleavage ; Kinetics of DNA-protein interactions ; Kinetics of DNA interactions studied by surface plasmon resonance spectroscopy ; Quantitative DNase I Kinetics Footprinting ; Real time fluorescence anisotropy for studying protein-DNA interactions ; Analysis of protein-DNA interactions in complex nucleoprotein assemblies ; Site-specific protein-DNA photocrosslinking ; Visualization of protein-DNA complexes by cryo em and on grids ; Characterization of T7 RNA polymerase protein-DNA interactions during the initiation and elongation phases