DNA Protein Interactions

Filter-Binding Assays, Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions, DNase I Footprinting, Footprinting with Exonuclease III, Hydroxyl Radical Footprinting, The Use of Diethyl Pyrocarbonate (DEPC) and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA, Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper, Uranyl Photofootprinting, Osmium Tetroxide Modification and the Study of DNA-Protein Interactions, Determination of a Transcription Factor-Binding Site by Nuclease Protection Footprinting Onto Southwestern Blots, Diffusible Singlet Oxygen as a Probe of DNA Deformation, UV-Laser Footprinting, Determination of Nucleic Acid Recognition Sequences by SELEX, Identification of Protein-DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference, Ethylation Interference, Hydroxyl Radical Interference, Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting, A Competition Assay for DNA Binding Using the Fluorescent Probe ANS, Site-Directed Cleavage of DNA Protein-Fe(II) EDTA Conjugates, Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes, Chemical Modification of Lysine by Reductive Methylation; a Probe of Residues Involved in DNA Binding, Limited Proteolysis of Protein-Nucleic Acid Complexes, Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine, Site-Specific Protein-DNA Photocrosslinking: Analysis of Bacterial Transcription Initiation Complexes, Site Directed DNA Photoaffinity Labeling of RNA Polymerase III Transcription Complexes, The Use of Site-Specific Protein-DNA Photo-Crosslinking to Analyze the Molecular Organization of the RNA Polymerase II Initiation Complex, UV Laser-Induced Protein-DNA Crosslinking, Plasmid Vectors for the Analysis of Protein-Induced DNA Bending, Engineering Nucleic Acid-Binding Proteins Genetic Analysis Of DNA-Protein Interactions Using A Reporter Gene Assay In Yeast, Assays for Transcription Factor Activity, Assay of Restriction Endonucleases Using Oligonucleotides, Analysis of DNA-Protein Interactions by Intrinsic Fluorescence, Circular Dichroism for the Analysis of Protein-DNA Interactions, Calorimetry of Protein-DNA Complexes and Their Components, Surface Plasmon Resonance Applied to DNA-Protein Complexes, Reconstitution of Protein-DNA Complexes for Crystallization, 2D Crystal Arrays in Protein Structure Studies, Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates, Electron Microscopy of Protein-Nucleic Acid Complexes: Uniform Spreading of Flexible Complexes, Staining With a Uniform Thin Layer of Uranyl Acetate and Determination of Helix Handedness, Scanning Transmission Electron Microscopy of DNA-Protein Complexes, High DNA-Protein Crosslinking Yield With Two-Wavelength Femtosecond (TWF) Laser Irradiation,