Tandem Repeats in Genes, Proteins, and Disease

Longitudinal Imaging and Analysis of Neurons Expressing Polyglutamine-Expanded Proteins.- Atomic Force Microscopy Assays for Evaluating Polyglutamine Aggregation in Solution and on Surfaces.- Morphometric Analysis of Huntington’s Disease Neurodegeneration in Drosophila.- Size Analysis of Polyglutamine Protein Aggregates using Fluorescence Detection in an Analytical Ultracentrifuge.- A Method for the Incremental Expansion of Polyglutamine Repeats in Recombinant Proteins.- Pulse Shape Analysis (PulSA) to Track Protein Translocalization in Cells by Flow Cytometry: Applications for Polyglutamine Aggregation.- Characterizing Social Behavior in Genetically Targeted Mouse Models of Brain Disorders.- PCR Amplification and Sequence Analysis of GC-rich Sequences: Aristaless-Related Homeobox Example.- Challenges of “sticky” Co-Immunoprecipitation; Polyalanine Tract Protein-Protein Interactions.- Molecular Pathology of Polyalanine Expansion Disorders-new Perspectives from Mouse Models.- Yeast as a Platform to Explore Polyglutamine Toxicity and Aggregation.- Immunobased Detection Assays to Quantify Distinct Mutant Huntingtin Conformations in Biological Samples.- Modeling and Analysis of Repeat RNA Toxicity in Drosophila.- Analyzing Modifiers of Protein Aggregation in C. elegans by Native Agarose Gel Electrophoresis.- Kinetic Analysis of Aggregation Data.- A Bioinformatics Method for Identifying Q/N-rich Prion-like Domains in Proteins.- Detecting Soluble PolyQ Oligomers and Investigating their Impact on Living Cells using Split-GFP.- Cell Biological Approaches To Investigate Polyglutamine-Expanded AR Metabolism.