Quantitative PCR Protocols

Part I. Reviews. Quantitative PCR: A Survey of the Present Technology, General Principles of Quantitative PCR, Effects of Collection, Processing, and Storage on RNA Detection and Quantification, Quantitative RT-PCR, Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard, Comparison of Competitive PCR and Positive Control-Based PCR, Part II. Protocols. End-Point Titration-PCR for Quantitation of Cytomegalovirus DNA, Analysis of Amplified DNA Molecules by Capillary Electrophoresis and Laser Induced Fluorescence, Competitor Calibration and Analysis of Competitive Amplified PCR Products by High-Performance Liquid Chromatography (HPLC), Quantifying Amplicons with ELISA, Competitive PCR Quantitation Utilizing a Microtiter Plate Based Format for the Detection of PCR Products, Competitive and Differential RT-PCR (CD-RT-PCR) for Measurement of Normalized Gene Expression Using Antisense Competitors, Amplified Assay for Specific Dual-Labeled DNA Using the Coagulation Cascade (EDNA-ELCA), Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Suppressor Gene, Quantitative Analysis of Human DNA Sequences by PCR and Solid-Phase Minisequencing, High Resolution PCR Quantitation by AmpliSensor Assay, Construction of Polycompetitors for Competitive PCR, Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA, A Stochastic PCR Approach for RNA Quantification in Multiple Samples, Quantitation of mRNA Species by RT-PCR on Total mRNA Population with Nonradioactive Probes, Index.