DNA Recombination

Methods to Study Mitotic Homologous Recombination and Genome Stability.- Characterizing Resection at Random and Unique Chromosome Double-Strand Breaks and Telomere Ends.- Characterization of Meiotic Recombination Initiation Sites Using Pulsed-field Gel Electrophoresis.- Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using Single-stranded DNA.- Detection of Covalent DNA-Bound Spo11 and Topoisomerase Complexes.- Molecular Assays to Investigate Chromatin Changes during DNA Double-Strand Break Repair in Yeast.- Analysis of Meiotic Recombination Intermediates by Two-Dimensional Gel Electrophoresis.- Mapping of Crossover Sites Using DNA Microarrays.- Using the Semi-Synthetic Epitope System to Identify Direct Substrates of the Meiosis-Specific Budding Yeast Kinase, Mek1.- Genetic and Molecular Analysis of Mitotic Recombination in Saccharomyces cerevisiae.- In vivo Site-Specific Mutagenesis and Gene Collage Using the Delitto Perfetto System in Yeast Saccharomyces cerevisiae.- Detection of RNA-Templated Double-Strand Break Repair in Yeast.- SNP-Based Mapping of Crossover Recombination in C. elegans.- Characterization of Meiotic Crossovers in Pollen from Arabidopsis thaliana.- Isolation of Meiotic Recombinants from Mouse Sperm.- Homologous Recombination Assay for Interstrand Crosslink Repair.- Evaluation of Homologous Recombinational Repair in Chicken B Lymphoma Cell Line, DT40.- Understanding the Immunoglobulin Locus Specificity of Hypermutation.- Quality Control of Purified Proteins Involved in Homologous Recombination.- Assays for Structure-Selective DNA Endonucleases.- In vitro Assays for DNA Pairing and  Recombination-Associated DNA Synthesis.- An In vitro Assay for Monitoring the Formation and Branch Migration of Holliday Junctions Mediated by aEukaryotic Recombinase.- Reconstituting the Key Steps of the DNA Double-Strand Break Repair In vitro.- Biochemical Studies on Human Rad51-Mediated Homologous Recombination.- Studying DNA Replication Fork Stability in Xenopus Egg Extract.- Supported Lipid-Bilayers and DNA Curtains for High-Throughput Single Molecule Studies.- FRET-Based Assays to Monitor DNA Binding and Annealing by Rad52 Recombination Mediator Protein.- Visualization of Human Dmc1 Presynaptic Filaments.- Tracking of Single and Multiple Genomic Loci in Living Yeast Cells.- Cell Biology of Homologous Recombination in Yeast.- Live Cell Imaging of Meiotic Chromosome Dynamics in Yeast.- Chromosome Structure and Homologous Chromosome Association during Meiotic Prophase in C. elegans